9gyb

Crystal structure of the recombinant CODH from Rhodopspirillum rubrum produced in Escherichia coliCrystal structure of the recombinant CODH from Rhodopspirillum rubrum produced in Escherichia coli

Structural highlights 9gyb is a 6 chain structure with sequence from Rhodospirillum rubrum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.9Å Ligands: Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function COOS_RHORU Allows growth in a CO-dependent manner in the dark. CODH oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (possibly CooH). Publication Abstract from PubMed The [NiFe]-CODH from Rhodospirillum rubrum contains [4Fe4S] clusters that allow electron transfer from the buried active sites to the protein surface. Among them, the role of the D-cluster, located at the dimer interface is still not fully understood. In this study, the removal of the D-cluster by site-directed mutagenesis revealed remarkable features in the behavior of the enzyme. Quantitative analysis and spectroscopic studies unveiled the suppression of D-cluster in the mutants and the influence on other metal cofactors. Furthermore, the CO oxidation activity in solution measured in the presence of methylviologen is almost completely abolished in the mutants. Conversely, direct electrochemistry at a functionalized carbon nanotube electrode shows that the mutants are still catalytically active reaching reduced but significant current densities of 0.7 mA cm(-2). Moreover, the electroenzymatic activity towards oxygen is not affected by the removal of the D cluster. EPR studies reveal a remarkable change in the magnetic coupling between FeS clusters upon removal of the D-cluster, highlighting the effect of the location of this D-cluster at the dimer interface. It is noteworthy that in addition to its role as electron relay, the D-cluster appears to play an important role in the biosynthesis of the active site. Insights into the Role of the D-Cluster in [NiFe]-CODH from Rhodospirillum Rubrum.,Contaldo U, Guigliarelli B, Perard J, Pichon T, Le Goff A, Cavazza C Chemistry. 2025 Feb 6:e202403648. doi: 10.1002/chem.202403648. PMID:39912726 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 9gyb, resolution 2.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image