9ev0
Structure of the AAP filament of Sulfolobus acidocaldarius strain MW039 (delta agl3 mutant).Structure of the AAP filament of Sulfolobus acidocaldarius strain MW039 (delta agl3 mutant).
Structural highlights
FunctionPublication Abstract from PubMedArchaea produce various protein filaments with specialised functions. While some archaea produce only one type of filament, the archaeal model species Sulfolobus acidocaldarius generates four. These include rotary swimming propellers analogous to bacterial flagella (archaella), pili for twitching motility (Aap), adhesive fibres (threads), and filaments facilitating homologous recombination upon UV stress (UV pili). Here, we use cryo-electron microscopy to describe the structure of the S. acidocaldarius archaellum at 2.0 A resolution, and update the structures of the thread and the Aap pilus at 2.7 A and 2.6 A resolution, respectively. We define features unique to archaella of the order Sulfolobales and compare their structure to those of Aap and threads in the context of the S-layer. We define distinct N-glycan patterns in the three filaments and identify a putative O-glycosylation site in the thread. Finally, we ascertain whether N-glycan truncation leads to structural changes in archaella and Aap. Towards a molecular picture of the archaeal cell surface.,Gaines MC, Isupov MN, McLaren M, Mollat CL, Haque RU, Stephenson JK, Sivabalasarma S, Hanus C, Kattnig D, Gold VAM, Albers S, Daum B Nat Commun. 2024 Nov 29;15(1):10401. doi: 10.1038/s41467-024-53986-9. PMID:39614099[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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