8zac

Crystal structure of BcABA3 from Botrytis cinereaCrystal structure of BcABA3 from Botrytis cinerea

Structural highlights

8zac is a 2 chain structure with sequence from Botrytis cinerea B05.10. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.45Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ABA3_BOTFB Alpha-ionylideneethane synthase; part of the gene cluster that mediates the biosynthesis of abscisic acid (ABA), a phytohormone that acts antagonistically toward salicylic acid (SA), jasmonic acid (JA) and ethylene (ETH) signaling, to impede plant defense responses (PubMed:15240257, PubMed:16820452). The first step of the pathway catalyzes the reaction from farnesyl diphosphate to alpha-ionylideneethane performed by the alpha-ionylideneethane synthase aba3 via a three-step reaction mechanism involving 2 neutral intermediates, beta-farnesene and allofarnesene (PubMed:30226766). The cytochrome P450 monooxygenase aba1 might then be involved in the conversion of alpha-ionylideneethane to alpha-ionylideneacetic acid (Probable). Alpha-ionylideneacetic acid is further converted to abscisic acid in 2 steps involving the cytochrome P450 monooxygenase aba2 and the short-chain dehydrogenase/reductase aba4, via the intermediates 1'-deoxy-ABA or 1',4'-trans-diol-ABA, depending on the order of action of these 2 enzymes (Probable). Aba2 is responsible for the hydroxylation of carbon atom C-1' and aba4 might be involved in the oxidation of the C-4' carbon atom (PubMed:16820452).^[1] ^[2] ^[3] ^[4]

References

↑ Siewers V, Smedsgaard J, Tudzynski P. The P450 monooxygenase BcABA1 is essential for abscisic acid biosynthesis in Botrytis cinerea. Appl Environ Microbiol. 2004 Jul;70(7):3868-76. PMID:15240257 doi:10.1128/AEM.70.7.3868-3876.2004
↑ Siewers V, Kokkelink L, Smedsgaard J, Tudzynski P. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea. Appl Environ Microbiol. 2006 Jul;72(7):4619-26. PMID:16820452 doi:10.1128/AEM.02919-05
↑ Takino J, Kozaki T, Sato Y, Liu C, Ozaki T, Minami A, Oikawa H. Unveiling Biosynthesis of the Phytohormone Abscisic Acid in Fungi: Unprecedented Mechanism of Core Scaffold Formation Catalyzed by an Unusual Sesquiterpene Synthase. J Am Chem Soc. 2018 Oct 3;140(39):12392-12395. PMID:30226766 doi:10.1021/jacs.8b08925
↑ Siewers V, Kokkelink L, Smedsgaard J, Tudzynski P. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea. Appl Environ Microbiol. 2006 Jul;72(7):4619-26. PMID:16820452 doi:10.1128/AEM.02919-05

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OCA

[1] Siewers V, Smedsgaard J, Tudzynski P. The P450 monooxygenase BcABA1 is essential for abscisic acid biosynthesis in Botrytis cinerea. Appl Environ Microbiol. 2004 Jul;70(7):3868-76. PMID:15240257 doi:10.1128/AEM.70.7.3868-3876.2004

[2] Siewers V, Kokkelink L, Smedsgaard J, Tudzynski P. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea. Appl Environ Microbiol. 2006 Jul;72(7):4619-26. PMID:16820452 doi:10.1128/AEM.02919-05

[3] Takino J, Kozaki T, Sato Y, Liu C, Ozaki T, Minami A, Oikawa H. Unveiling Biosynthesis of the Phytohormone Abscisic Acid in Fungi: Unprecedented Mechanism of Core Scaffold Formation Catalyzed by an Unusual Sesquiterpene Synthase. J Am Chem Soc. 2018 Oct 3;140(39):12392-12395. PMID:30226766 doi:10.1021/jacs.8b08925

[4] Siewers V, Kokkelink L, Smedsgaard J, Tudzynski P. Identification of an abscisic acid gene cluster in the grey mold Botrytis cinerea. Appl Environ Microbiol. 2006 Jul;72(7):4619-26. PMID:16820452 doi:10.1128/AEM.02919-05

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