8p5o

Proline activating adenylation domain of gramicidin S synthetase 2 - GrsB1-AcoreProline activating adenylation domain of gramicidin S synthetase 2 - GrsB1-Acore

Structural highlights 8p5o is a 4 chain structure with sequence from Aneurinibacillus migulanus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.6Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Engineering of biosynthetic enzymes is increasingly employed to synthesize structural analogues of antibiotics. Of special interest are nonribosomal peptide synthetases (NRPSs) responsible for the production of important antimicrobial peptides. Here, directed evolution of an adenylation domain of a Pro-specific NRPS module completely switched substrate specificity to the non-standard amino acid piperazic acid (Piz) bearing a labile N-N bond. This success was achieved by UPLC-MS/MS-based screening of small, rationally designed mutant libraries and can presumably be replicated with a larger number of substrates and NRPS modules. The evolved NRPS produces a Piz-derived gramicidin S analogue. Thus, we give new impetus to the too-early dismissed idea that widely accessible low-throughput methods can switch the specificity of NRPSs in a biosynthetically useful fashion. Directed Evolution of Piperazic Acid Incorporation by a Nonribosomal Peptide Synthetase.,Stephan P, Langley C, Winkler D, Basquin J, Caputi L, O'Connor SE, Kries H Angew Chem Int Ed Engl. 2023 Aug 28;62(35):e202304843. doi: , 10.1002/anie.202304843. Epub 2023 Jul 18. PMID:37326625 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 8p5o, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image