8k10

SID1 transmembrane family member 2SID1 transmembrane family member 2

Structural highlights

8k10 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SIDT2_HUMAN Mediates the translocation of RNA and DNA across the lysosomal membrane during RNA and DNA autophagy (RDA), a process in which RNA or DNA is directly imported into lysosomes in an ATP-dependent manner, and degraded (PubMed:27046251, PubMed:27846365). Involved in the uptake of single-stranded oligonucleotides by living cells, a process called gymnosis (PubMed:28277980). In vitro, mediates the uptake of linear DNA more efficiently than that of circular DNA, but exhibits similar uptake efficacy toward RNA and DNA. Binds long double-stranded RNA (dsRNA) (500 - 700 base pairs), but not dsRNA shorter than 100 bp (By similarity).[UniProtKB:Q8CIF6]^[1] ^[2] ^[3]

References

↑ Aizawa S, Fujiwara Y, Contu VR, Hase K, Takahashi M, Kikuchi H, Kabuta C, Wada K, Kabuta T. Lysosomal putative RNA transporter SIDT2 mediates direct uptake of RNA by lysosomes. Autophagy. 2016;12(3):565-78. PMID:27046251 doi:10.1080/15548627.2016.1145325
↑ Aizawa S, Contu VR, Fujiwara Y, Hase K, Kikuchi H, Kabuta C, Wada K, Kabuta T. Lysosomal membrane protein SIDT2 mediates the direct uptake of DNA by lysosomes. Autophagy. 2017 Jan 2;13(1):218-222. PMID:27846365 doi:10.1080/15548627.2016.1248019
↑ Takahashi M, Contu VR, Kabuta C, Hase K, Fujiwara Y, Wada K, Kabuta T. SIDT2 mediates gymnosis, the uptake of naked single-stranded oligonucleotides into living cells. RNA Biol. 2017 Nov 2;14(11):1534-1543. PMID:28277980 doi:10.1080/15476286.2017.1302641

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OCA

[1] Aizawa S, Fujiwara Y, Contu VR, Hase K, Takahashi M, Kikuchi H, Kabuta C, Wada K, Kabuta T. Lysosomal putative RNA transporter SIDT2 mediates direct uptake of RNA by lysosomes. Autophagy. 2016;12(3):565-78. PMID:27046251 doi:10.1080/15548627.2016.1145325

[2] Aizawa S, Contu VR, Fujiwara Y, Hase K, Kikuchi H, Kabuta C, Wada K, Kabuta T. Lysosomal membrane protein SIDT2 mediates the direct uptake of DNA by lysosomes. Autophagy. 2017 Jan 2;13(1):218-222. PMID:27846365 doi:10.1080/15548627.2016.1248019

[3] Takahashi M, Contu VR, Kabuta C, Hase K, Fujiwara Y, Wada K, Kabuta T. SIDT2 mediates gymnosis, the uptake of naked single-stranded oligonucleotides into living cells. RNA Biol. 2017 Nov 2;14(11):1534-1543. PMID:28277980 doi:10.1080/15476286.2017.1302641

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