8jd8

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Crystal structure of Citrus limon Cu-Zn superoxide dismutaseCrystal structure of Citrus limon Cu-Zn superoxide dismutase

Structural highlights

8jd8 is a 4 chain structure with sequence from Citrus limon. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.86Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q71S31_CITLI Destroys radicals which are normally produced within the cells and which are toxic to biological systems.[RuleBase:RU000393]

Publication Abstract from PubMed

The copper-zinc superoxide dismutase (CuZnSOD) from lemon (SOD_CL) is active in an acidic environment and resists proteolytic degradation. The enzyme occurs as a dimer, which has an indirect effect on the enzyme activity as the monomer retains only approximately 35% of the activity. Here, the crystal structure of SOD_CL at 1.86 A resolution is reported that may explain this peculiarity. The crystal belonged to space group P2(1), with unit-cell parameters a = 61.11, b = 74.55, c = 61.69 A, beta = 106.86 degrees , and contained four molecules in the asymmetric unit. The overall structure of SOD_CL resembles that of CuZnSOD from plants. The structure of SOD_CL shows a unique arrangement of surface loop IV that connects the dimer interface and the active site, which is located away from the dimer-interface region. This arrangement allows direct interaction between the residues residing in the dimer interface and those in the active site. The arrangement also includes Leu62 and Gln164, which are conserved in cytoplasmic CuZnSOD. This supports the classification of SOD_CL as a cytoplasmic CuZnSOD despite sharing the highest amino-acid sequence homology with CuZnSODs from spinach and tomato, which are chloroplastic.

Direct relationship between dimeric form and activity in the acidic copper-zinc superoxide dismutase from lemon.,Utami RA, Yoshida H, Kartadinata LH, Abdillah VA, Faratilla CR, Retnoningrum DS, Ismaya WT Acta Crystallogr F Struct Biol Commun. 2023 Dec 1;79(Pt 12):301-307. doi: , 10.1107/S2053230X23010646. Epub 2023 Dec 18. PMID:38108885[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Utami RA, Yoshida H, Kartadinata LH, Abdillah VA, Faratilla CR, Retnoningrum DS, Ismaya WT. Direct relationship between dimeric form and activity in the acidic copper-zinc superoxide dismutase from lemon. Acta Crystallogr F Struct Biol Commun. 2023 Dec 1;79(Pt 12):301-307. PMID:38108885 doi:10.1107/S2053230X23010646

8jd8, resolution 1.86Å

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