Crystal structure of ApNGT with Q469A and M218A mutations in complex with UDP-GLCCrystal structure of ApNGT with Q469A and M218A mutations in complex with UDP-GLC

Structural highlights

8j30 is a 2 chain structure with sequence from Actinobacillus pleuropneumoniae serovar 5b str. L20. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.89Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NGT_ACTP2 Inverting glycosyltransferase that catalyzes the transfer of one glucose moiety from UDP-glucose to an asparagine residue in peptides and proteins containing the NX(S/T) motif, resulting in their modification with a beta-linked 1,N-glucose. Likely acts as a key component of a general protein glycosylation system (Probable).[1]

References

  1. Schwarz F, Fan YY, Schubert M, Aebi M. Cytoplasmic N-glycosyltransferase of Actinobacillus pleuropneumoniae is an inverting enzyme and recognizes the NX(S/T) consensus sequence. J Biol Chem. 2011 Oct 7;286(40):35267-74. PMID:21852240 doi:10.1074/jbc.M111.277160

8j30, resolution 2.89Å

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