Structure of neck with portal vertex of capsid of Agrobacterium phage MilanoStructure of neck with portal vertex of capsid of Agrobacterium phage Milano

Structural highlights

8fxr is a 202 chain structure with sequence from Agrobacterium phage Milano. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Electron Microscopy, Resolution 4.5Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A482MHL8_9CAUD

Publication Abstract from PubMed

Large gaps exist in our understanding of how bacteriophages, the most abundant biological entities on Earth, assemble and function. The structure of the "neck" region, where the DNA-filled capsid is connected to the host-recognizing tail remains poorly understood. We describe cryo-EM structures of the neck, the neck-capsid and neck-tail junctions, and capsid of the Agrobacterium phage Milano. The Milano neck 1 protein connects the 12-fold symmetrical neck to a 5-fold vertex of the icosahedral capsid. Comparison of Milano neck 1 homologs leads to four proposed classes, likely evolved from the simplest one in siphophages to more complex ones in myo- and podophages. Milano neck is surrounded by the atypical collar, which covalently crosslinks the tail sheath to neck 1. The Milano capsid is decorated with three types of proteins, a minor capsid protein (mCP) and two linking proteins crosslinking the mCP to the major capsid protein. The extensive network of disulfide bonds within and between neck, collar, capsid and tail provides an exceptional structural stability to Milano.

Neck and capsid architecture of the robust Agrobacterium phage Milano.,Sonani RR, Esteves NC, Horton AA, Kelly RJ, Sebastian AL, Wang F, Kreutzberger MAB, Leiman PG, Scharf BE, Egelman EH Commun Biol. 2023 Sep 8;6(1):921. doi: 10.1038/s42003-023-05292-1. PMID:37684529[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Sonani RR, Esteves NC, Horton AA, Kelly RJ, Sebastian AL, Wang F, Kreutzberger MAB, Leiman PG, Scharf BE, Egelman EH. Neck and capsid architecture of the robust Agrobacterium phage Milano. Commun Biol. 2023 Sep 8;6(1):921. PMID:37684529 doi:10.1038/s42003-023-05292-1

8fxr, resolution 4.50Å

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