8cr7
Crystal structure of recombinant LasB from Pseudomonas aeruginosa PA7Crystal structure of recombinant LasB from Pseudomonas aeruginosa PA7
Structural highlights
FunctionELAS_PSEAE Cleaves elastin, collagen, IgG, and several complement components. Involved in the pathogenesis of P.aeruginosa infections. Publication Abstract from PubMedThe human pathogen Pseudomonas aeruginosa has a number of virulence factors at its disposal that play crucial roles in the progression of infection. LasB is one of the major virulence factors and exerts its effects through elastolytic and proteolytic activities aimed at dissolving connective tissue and inactivating host defense proteins. LasB is of great interest for the development of novel pathoblockers to temper the virulence, but access has thus far largely been limited to protein isolated from Pseudomonas cultures. Here, we describe a new protocol for high-level production of native LasB in Escherichia coli. We demonstrate that this facile approach is suitable for the production of mutant, thus far inaccessible LasB variants, and characterize the proteins biochemically and structurally. We expect that easy access to LasB will accelerate the development of inhibitors for this important virulence factor. Facile Production of the Pseudomonas aeruginosa Virulence Factor LasB in Escherichia coli for Structure-Based Drug Design.,Kolling D, Haupenthal J, Hirsch AKH, Koehnke J Chembiochem. 2023 Sep 1;24(17):e202300185. doi: 10.1002/cbic.202300185. Epub 2023 , Jul 31. PMID:37195753[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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