7xkx

Crystal structure of Tpn2Crystal structure of Tpn2

Structural highlights 7xkx is a 2 chain structure with sequence from Kitasatospora sp. CB02891. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.57Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A2M9LDX2_9ACTN Publication Abstract from PubMed A grand challenge in terpene synthase (TS) enzymology is the ability to predict function from protein sequence. Given the limited number of characterized bacterial TSs and significant sequence diversities between them and their eukaryotic counterparts, this is currently impossible. To contribute towards understanding the sequence-structure-function relationships of type II bacterial TSs, we determined the structure of the terpentedienyl diphosphate synthase Tpn2 from Kitasatospora sp. CB02891 by X-ray crystallography and made structure-guided mutants to probe its mechanism. Substitution of a glycine into a basic residue changed the product preference from the clerodane skeleton to a syn-labdane skeleton, resulting in the first syn-labdane identified from a bacterial TS. Understanding how a single residue can dictate the cyclization pattern in Tpn2, along with detailed bioinformatics analysis of bacterial type II TSs, sets the stage for the investigation of the functional scope of bacterial type II TSs and the discovery of novel bacterial terpenoids. Structure-guided product determination of the bacterial type II diterpene synthase Tpn2.,Stowell EA, Ehrenberger MA, Lin YL, Chang CY, Rudolf JD Commun Chem. 2022 Nov 8;5(1):146. doi: 10.1038/s42004-022-00765-6. PMID:36698006 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 7xkx, resolution 2.57Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image