7wb8

Crystal structure of Bovine Pancreatic Trypsin in complex with 5-Methoxytryptamine at Room TemperatureCrystal structure of Bovine Pancreatic Trypsin in complex with 5-Methoxytryptamine at Room Temperature

Structural highlights

7wb8 is a 1 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.38Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TRY1_BOVIN

Publication Abstract from PubMed

In situ diffraction data collection using crystallization plates has been utilized for macromolecules to evaluate crystal quality without requiring additional sample treatment such as cryocooling. Although it is difficult to collect complete data sets using this technique due to the mechanical limitation of crystal rotation, recent advances in methods for data collection from multiple crystals have overcome this issue. At SPring-8, an in situ diffraction measurement system was constructed consisting of a goniometer for a plate, an articulated robot and plate storage. Using this system, complete data sets were obtained utilizing the small-wedge measurement method. Combining this system with an acoustic liquid handler to prepare protein-ligand complex crystals by applying fragment compounds to trypsin crystals for in situ soaking, binding was confirmed for seven out of eight compounds. These results show that the system functioned properly to collect complete data for structural analysis and to expand the capability for ligand screening in combination with a liquid dispenser.

In situ crystal data-collection and ligand-screening system at SPring-8.,Okumura H, Sakai N, Murakami H, Mizuno N, Nakamura Y, Ueno G, Masunaga T, Kawamura T, Baba S, Hasegawa K, Yamamoto M, Kumasaka T Acta Crystallogr F Struct Biol Commun. 2022 Jun 1;78(Pt 6):241-251. doi: , 10.1107/S2053230X22005283. Epub 2022 May 27. PMID:35647681^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Okumura H, Sakai N, Murakami H, Mizuno N, Nakamura Y, Ueno G, Masunaga T, Kawamura T, Baba S, Hasegawa K, Yamamoto M, Kumasaka T. In situ crystal data-collection and ligand-screening system at SPring-8. Acta Crystallogr F Struct Biol Commun. 2022 Jun 1;78(Pt 6):241-251. PMID:35647681 doi:10.1107/S2053230X22005283

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OCA

[1] Okumura H, Sakai N, Murakami H, Mizuno N, Nakamura Y, Ueno G, Masunaga T, Kawamura T, Baba S, Hasegawa K, Yamamoto M, Kumasaka T. In situ crystal data-collection and ligand-screening system at SPring-8. Acta Crystallogr F Struct Biol Commun. 2022 Jun 1;78(Pt 6):241-251. PMID:35647681 doi:10.1107/S2053230X22005283

[1]