7r9f

Wild-type yeast Pseudouridine Synthase, PUS1, bound to 5-Fluorouracil RNAWild-type yeast Pseudouridine Synthase, PUS1, bound to 5-Fluorouracil RNA

Structural highlights

7r9f is a 2 chain structure with sequence from Saccharomyces cerevisiae and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.89Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PUS1_YEAST Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr) (PubMed:9671058, PubMed:10356324, PubMed:25219674). Catalyzes pseudouridylation at position 44 in U2 snRNA (PubMed:25219674). Also catalyzes pseudouridylation of mRNAs (PubMed:25219674, PubMed:31477916).^[1] ^[2] ^[3] ^[4]

References

↑ Arluison V, Buckle M, Grosjean H. Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA. J Mol Biol. 1999 Jun 11;289(3):491-502. PMID:10356324 doi:10.1006/jmbi.1999.2789
↑ Schwartz S, Bernstein DA, Mumbach MR, Jovanovic M, Herbst RH, León-Ricardo BX, Engreitz JM, Guttman M, Satija R, Lander ES, Fink G, Regev A. Transcriptome-wide mapping reveals widespread dynamic-regulated pseudouridylation of ncRNA and mRNA. Cell. 2014 Sep 25;159(1):148-162. PMID:25219674 doi:10.1016/j.cell.2014.08.028
↑ Carlile TM, Martinez NM, Schaening C, Su A, Bell TA, Zinshteyn B, Gilbert WV. mRNA structure determines modification by pseudouridine synthase 1. Nat Chem Biol. 2019 Oct;15(10):966-974. doi: 10.1038/s41589-019-0353-z. Epub 2019, Sep 2. PMID:31477916 doi:http://dx.doi.org/10.1038/s41589-019-0353-z
↑ Motorin Y, Keith G, Simon C, Foiret D, Simos G, Hurt E, Grosjean H. The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity. RNA. 1998 Jul;4(7):856-69. PMID:9671058 doi:10.1017/s1355838298980396

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OCA

[1] Arluison V, Buckle M, Grosjean H. Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA. J Mol Biol. 1999 Jun 11;289(3):491-502. PMID:10356324 doi:10.1006/jmbi.1999.2789

[2] Schwartz S, Bernstein DA, Mumbach MR, Jovanovic M, Herbst RH, León-Ricardo BX, Engreitz JM, Guttman M, Satija R, Lander ES, Fink G, Regev A. Transcriptome-wide mapping reveals widespread dynamic-regulated pseudouridylation of ncRNA and mRNA. Cell. 2014 Sep 25;159(1):148-162. PMID:25219674 doi:10.1016/j.cell.2014.08.028

[3] Carlile TM, Martinez NM, Schaening C, Su A, Bell TA, Zinshteyn B, Gilbert WV. mRNA structure determines modification by pseudouridine synthase 1. Nat Chem Biol. 2019 Oct;15(10):966-974. doi: 10.1038/s41589-019-0353-z. Epub 2019, Sep 2. PMID:31477916 doi:http://dx.doi.org/10.1038/s41589-019-0353-z

[4] Motorin Y, Keith G, Simon C, Foiret D, Simos G, Hurt E, Grosjean H. The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity. RNA. 1998 Jul;4(7):856-69. PMID:9671058 doi:10.1017/s1355838298980396

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