7nds

Crystal structure of TphC in a closed conformationCrystal structure of TphC in a closed conformation

Structural highlights 7nds is a 1 chain structure with sequence from Comamonas sp.. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.4Å Ligands: Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A5N7XFM8_COMSP Publication Abstract from PubMed Biological degradation of Polyethylene terephthalate (PET) plastic and assimilation of the corresponding monomers ethylene glycol and terephthalate (TPA) into central metabolism offers an attractive route for bio-based molecular recycling and bioremediation applications. A key step is the cellular uptake of the non-permeable TPA into bacterial cells which has been shown to be dependent upon the presence of the key tphC gene. However, little is known from a biochemical and structural perspective about the encoded solute binding protein, TphC. Here, we report the biochemical and structural characterisation of TphC in both open and TPA-bound closed conformations. This analysis demonstrates the narrow ligand specificity of TphC towards aromatic para-substituted dicarboxylates, such as TPA and closely related analogues. Further phylogenetic and genomic context analysis of the tph genes reveals homologous operons as a genetic resource for future biotechnological and metabolic engineering efforts towards circular plastic bio-economy solutions. Structural basis of terephthalate recognition by solute binding protein TphC.,Gautom T, Dheeman D, Levy C, Butterfield T, Alvarez Gonzalez G, Le Roy P, Caiger L, Fisher K, Johannissen L, Dixon N Nat Commun. 2021 Oct 29;12(1):6244. doi: 10.1038/s41467-021-26508-0. PMID:34716322 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 7nds, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image