7dl9

Crystal structure of nucleoside transporter NupGCrystal structure of nucleoside transporter NupG

Structural highlights

7dl9 is a 2 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NUPG_ECOLI Broad-specificity transporter of purine and pyrimidine nucleosides. Driven by a proton motive force. Can transport uridine, adenosine, inosine, guanosine, thymidine and cytidine. Can also transport xanthosine, but with a very low affinity.^[1] ^[2] ^[3] ^[4] ^[5]

References

↑ Norholm MH, Dandanell G. Specificity and topology of the Escherichia coli xanthosine permease, a representative of the NHS subfamily of the major facilitator superfamily. J Bacteriol. 2001 Aug;183(16):4900-4. doi: 10.1128/JB.183.16.4900-4904.2001. PMID:11466294 doi:http://dx.doi.org/10.1128/JB.183.16.4900-4904.2001
↑ Xie H, Patching SG, Gallagher MP, Litherland GJ, Brough AR, Venter H, Yao SY, Ng AM, Young JD, Herbert RB, Henderson PJ, Baldwin SA. Purification and properties of the Escherichia coli nucleoside transporter NupG, a paradigm for a major facilitator transporter sub-family. Mol Membr Biol. 2004 Sep-Oct;21(5):323-36. doi: 10.1080/09687860400003941. PMID:15513740 doi:http://dx.doi.org/10.1080/09687860400003941
↑ Patching SG, Baldwin SA, Baldwin AD, Young JD, Gallagher MP, Henderson PJ, Herbert RB. The nucleoside transport proteins, NupC and NupG, from Escherichia coli: specific structural motifs necessary for the binding of ligands. Org Biomol Chem. 2005 Feb 7;3(3):462-70. doi: 10.1039/b414739a. Epub 2005 Jan 10. PMID:15678184 doi:http://dx.doi.org/10.1039/b414739a
↑ Westh Hansen SE, Jensen N, Munch-Petersen A. Studies on the sequence and structure of the Escherichia coli K-12 nupG gene, encoding a nucleoside-transport system. Eur J Biochem. 1987 Oct 15;168(2):385-91. doi:, 10.1111/j.1432-1033.1987.tb13431.x. PMID:3311747 doi:http://dx.doi.org/10.1111/j.1432-1033.1987.tb13431.x
↑ Munch-Petersen A, Mygind B, Nicolaisen A, Pihl NJ. Nucleoside transport in cells and membrane vesicles from Escherichia coli K12. J Biol Chem. 1979 May 25;254(10):3730-7. PMID:374403

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OCA

[1] Norholm MH, Dandanell G. Specificity and topology of the Escherichia coli xanthosine permease, a representative of the NHS subfamily of the major facilitator superfamily. J Bacteriol. 2001 Aug;183(16):4900-4. doi: 10.1128/JB.183.16.4900-4904.2001. PMID:11466294 doi:http://dx.doi.org/10.1128/JB.183.16.4900-4904.2001

[2] Xie H, Patching SG, Gallagher MP, Litherland GJ, Brough AR, Venter H, Yao SY, Ng AM, Young JD, Herbert RB, Henderson PJ, Baldwin SA. Purification and properties of the Escherichia coli nucleoside transporter NupG, a paradigm for a major facilitator transporter sub-family. Mol Membr Biol. 2004 Sep-Oct;21(5):323-36. doi: 10.1080/09687860400003941. PMID:15513740 doi:http://dx.doi.org/10.1080/09687860400003941

[3] Patching SG, Baldwin SA, Baldwin AD, Young JD, Gallagher MP, Henderson PJ, Herbert RB. The nucleoside transport proteins, NupC and NupG, from Escherichia coli: specific structural motifs necessary for the binding of ligands. Org Biomol Chem. 2005 Feb 7;3(3):462-70. doi: 10.1039/b414739a. Epub 2005 Jan 10. PMID:15678184 doi:http://dx.doi.org/10.1039/b414739a

[4] Westh Hansen SE, Jensen N, Munch-Petersen A. Studies on the sequence and structure of the Escherichia coli K-12 nupG gene, encoding a nucleoside-transport system. Eur J Biochem. 1987 Oct 15;168(2):385-91. doi:, 10.1111/j.1432-1033.1987.tb13431.x. PMID:3311747 doi:http://dx.doi.org/10.1111/j.1432-1033.1987.tb13431.x

[5] Munch-Petersen A, Mygind B, Nicolaisen A, Pihl NJ. Nucleoside transport in cells and membrane vesicles from Escherichia coli K12. J Biol Chem. 1979 May 25;254(10):3730-7. PMID:374403

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