7cb6
The silver-bound 6-phosphogluconate dehydrogenase from Staphylococcus aureus (strain Newman)The silver-bound 6-phosphogluconate dehydrogenase from Staphylococcus aureus (strain Newman)
Structural highlights
FunctionA0A0H3KGN1_STAAE Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.[PIRNR:PIRNR000109] Publication Abstract from PubMedThe rapid emergence of drug resistant Staphylococcus aureus (S. aureus) poses a serious threat to public health globally. Silver (Ag)-based antimicrobials are promising to combat antibiotic resistant S. aureus, yet their molecular targets are largely elusive. Herein, we separate and identify 38 authentic Ag(+)-binding proteins in S. aureus at the whole-cell scale. We then capture the molecular snapshot on the dynamic action of Ag(+) against S. aureus and further validate that Ag(+) could inhibit a key target 6-phosphogluconate dehydrogenase through binding to catalytic His185 by X-ray crystallography. Significantly, the multi-target mode of action of Ag(+) (and nanosilver) endows its sustainable antimicrobial efficacy, leading to enhanced efficacy of conventional antibiotics and resensitization of MRSA to antibiotics. Our study resolves the long-standing question of the molecular targets of silver in S. aureus and offers insights into the sustainable bacterial susceptibility of silver, providing a potential approach for combating antimicrobial resistance. Multi-target mode of action of silver against Staphylococcus aureus endows it with capability to combat antibiotic resistance.,Wang H, Wang M, Xu X, Gao P, Xu Z, Zhang Q, Li H, Yan A, Kao RY, Sun H Nat Commun. 2021 Jun 7;12(1):3331. doi: 10.1038/s41467-021-23659-y. PMID:34099682[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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