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E.coli beta-galactosidase (E537Q) in complex with fluorescent probe KSA01E.coli beta-galactosidase (E537Q) in complex with fluorescent probe KSA01
Structural highlights
FunctionPublication Abstract from PubMedThe tracking of cellular senescence usually depends on the detection of senescence-associated beta-galactosidase (SA-beta-gal). Previous probes for SA-beta-gal with this purpose only cover a single dimension: the accumulation of this enzyme in lysosomes. However, this is insufficient to determine the destiny of senescence because endogenous beta-gal enriched in lysosomes is not only related to senescence, but also to some other physiological processes. To address this issue, we introduce our fluorescent probes including a second dimension: lysosomal pH, since de-acidification is a unique feature of the lysosomes in senescent cells. With this novel design, our probes achieved excellent discrimination of SA-beta-gal from cancer-associated beta-gal, which enables them to track cellular senescence as well as tissue aging more precisely. Our crystal structures of a model enzyme E. coli beta-gal mutant (E537Q) complexed with each probe further revealed the structural basis for probe recognition. Two-Dimensional Design Strategy to Construct Smart Fluorescent Probes for the Precise Tracking of Senescence.,Guo Y, Gao Y, Hu Y, Liu Q, Li X, Li X, Kim CY, James TD, Li J, Chen X Angew Chem Int Ed Engl. 2021 Feb 24. doi: 10.1002/anie.202101278. PMID:33624914[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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