7bm2

Crystal structure of the DNA-binding protein RemA from Geobacillus thermodenitrificansCrystal structure of the DNA-binding protein RemA from Geobacillus thermodenitrificans

Structural highlights 7bm2 is a 4 chain structure with sequence from Geotn. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NonStd Res: Gene: GTNG_1019 (GEOTN) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Bacillus subtilis can form structurally complex biofilms on solid or liquid surfaces, which requires expression of genes for matrix production. The transcription of these genes is activated by regulatory protein RemA, which binds to poorly conserved, repetitive DNA regions but lacks obvious DNA-binding motifs or domains. Here, we present the structure of the RemA homologue from Geobacillus thermodenitrificans, showing a unique octameric ring with the potential to form a 16-meric superstructure. These results, together with further biochemical and in vivo characterization of B. subtilis RemA, suggests that the protein can wrap DNA around its ring-like structure through a LytTR-related domain. Structural and functional characterization of the bacterial biofilm activator RemA.,Hoffmann T, Mrusek D, Bedrunka P, Burchert F, Mais CN, Kearns DB, Altegoer F, Bremer E, Bange G Nat Commun. 2021 Sep 29;12(1):5707. doi: 10.1038/s41467-021-26005-4. PMID:34588455 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 7bm2, resolution 2.29Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image