6zm6
Human mitochondrial ribosome in complex with mRNA, A/A tRNA and P/P tRNAHuman mitochondrial ribosome in complex with mRNA, A/A tRNA and P/P tRNA
Structural highlights
DiseaseRM03_HUMAN Combined oxidative phosphorylation defect type 9. The disease is caused by mutations affecting the gene represented in this entry. FunctionPublication Abstract from PubMedMitochondrial ribosomes (mitoribosomes) are tethered to the mitochondrial inner membrane to facilitate the cotranslational membrane insertion of the synthesized proteins. We report cryo-electron microscopy structures of human mitoribosomes with nascent polypeptide, bound to the insertase oxidase assembly 1-like (OXA1L) through three distinct contact sites. OXA1L binding is correlated with a series of conformational changes in the mitoribosomal large subunit that catalyze the delivery of newly synthesized polypeptides. The mechanism relies on the folding of mL45 inside the exit tunnel, forming two specific constriction sites that would limit helix formation of the nascent chain. A gap is formed between the exit and the membrane, making the newly synthesized proteins accessible. Our data elucidate the basis by which mitoribosomes interact with the OXA1L insertase to couple protein synthesis and membrane delivery. Mechanism of membrane-tethered mitochondrial protein synthesis.,Itoh Y, Andrell J, Choi A, Richter U, Maiti P, Best RB, Barrientos A, Battersby BJ, Amunts A Science. 2021 Feb 19;371(6531):846-849. doi: 10.1126/science.abe0763. PMID:33602856[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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