6wgi

Atomic model of the mutant OCCM (ORC-Cdc6-Cdt1-Mcm2-7 with Mcm6 WHD truncation) loaded on DNA at 10.5 A resolutionAtomic model of the mutant OCCM (ORC-Cdc6-Cdt1-Mcm2-7 with Mcm6 WHD truncation) loaded on DNA at 10.5 A resolution

Structural highlights

6wgi is a 10 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 10Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CDC6_YEAST Plays a crucial role in forming the pre-replicative complexes. Interacts with the origin recognition complex (ORC) and MCM2-7 helicase complex leading to the linking of those complexes and loading of the replicative helicase MCM2-7 onto the pre-replicative complexes. Required for the initiation of DNA replication and then actively participates in the suppression of nuclear division.^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7] ^[8] ^[9]

References

↑ Wang B, Feng L, Hu Y, Huang SH, Reynolds CP, Wu L, Jong AY. The essential role of Saccharomyces cerevisiae CDC6 nucleotide-binding site in cell growth, DNA synthesis, and Orc1 association. J Biol Chem. 1999 Mar 19;274(12):8291-8. PMID:10075735
↑ Bueno A, Russell P. Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division. EMBO J. 1992 Jun;11(6):2167-76. PMID:1600944
↑ Fernandez-Cid A, Riera A, Tognetti S, Herrera MC, Samel S, Evrin C, Winkler C, Gardenal E, Uhle S, Speck C. An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly. Mol Cell. 2013 May 23;50(4):577-88. doi: 10.1016/j.molcel.2013.03.026. Epub 2013 , Apr 18. PMID:23603117 doi:http://dx.doi.org/10.1016/j.molcel.2013.03.026
↑ Piatti S, Lengauer C, Nasmyth K. Cdc6 is an unstable protein whose de novo synthesis in G1 is important for the onset of S phase and for preventing a 'reductional' anaphase in the budding yeast Saccharomyces cerevisiae. EMBO J. 1995 Aug 1;14(15):3788-99. PMID:7641697
↑ Cocker JH, Piatti S, Santocanale C, Nasmyth K, Diffley JF. An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast. Nature. 1996 Jan 11;379(6561):180-2. PMID:8538771 doi:http://dx.doi.org/10.1038/379180a0
↑ Santocanale C, Diffley JF. ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae. EMBO J. 1996 Dec 2;15(23):6671-9. PMID:8978693
↑ Detweiler CS, Li JJ. Cdc6p establishes and maintains a state of replication competence during G1 phase. J Cell Sci. 1997 Mar;110 ( Pt 6):753-63. PMID:9099949
↑ Drury LS, Perkins G, Diffley JF. The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast. EMBO J. 1997 Oct 1;16(19):5966-76. PMID:9312054 doi:10.1093/emboj/16.19.5966
↑ Weinreich M, Liang C, Stillman B. The Cdc6p nucleotide-binding motif is required for loading mcm proteins onto chromatin. Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):441-6. PMID:9892652

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OCA

[1] Wang B, Feng L, Hu Y, Huang SH, Reynolds CP, Wu L, Jong AY. The essential role of Saccharomyces cerevisiae CDC6 nucleotide-binding site in cell growth, DNA synthesis, and Orc1 association. J Biol Chem. 1999 Mar 19;274(12):8291-8. PMID:10075735

[2] Bueno A, Russell P. Dual functions of CDC6: a yeast protein required for DNA replication also inhibits nuclear division. EMBO J. 1992 Jun;11(6):2167-76. PMID:1600944

[3] Fernandez-Cid A, Riera A, Tognetti S, Herrera MC, Samel S, Evrin C, Winkler C, Gardenal E, Uhle S, Speck C. An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly. Mol Cell. 2013 May 23;50(4):577-88. doi: 10.1016/j.molcel.2013.03.026. Epub 2013 , Apr 18. PMID:23603117 doi:http://dx.doi.org/10.1016/j.molcel.2013.03.026

[4] Piatti S, Lengauer C, Nasmyth K. Cdc6 is an unstable protein whose de novo synthesis in G1 is important for the onset of S phase and for preventing a 'reductional' anaphase in the budding yeast Saccharomyces cerevisiae. EMBO J. 1995 Aug 1;14(15):3788-99. PMID:7641697

[5] Cocker JH, Piatti S, Santocanale C, Nasmyth K, Diffley JF. An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast. Nature. 1996 Jan 11;379(6561):180-2. PMID:8538771 doi:http://dx.doi.org/10.1038/379180a0

[6] Santocanale C, Diffley JF. ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae. EMBO J. 1996 Dec 2;15(23):6671-9. PMID:8978693

[7] Detweiler CS, Li JJ. Cdc6p establishes and maintains a state of replication competence during G1 phase. J Cell Sci. 1997 Mar;110 ( Pt 6):753-63. PMID:9099949

[8] Drury LS, Perkins G, Diffley JF. The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast. EMBO J. 1997 Oct 1;16(19):5966-76. PMID:9312054 doi:10.1093/emboj/16.19.5966

[9] Weinreich M, Liang C, Stillman B. The Cdc6p nucleotide-binding motif is required for loading mcm proteins onto chromatin. Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):441-6. PMID:9892652

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