6jpu

CryoEM structure of Abo1 hexamer - apo complexCryoEM structure of Abo1 hexamer - apo complex

6jpu, resolution 4.27Å

Structural highlights

6jpu is a 6 chain structure with sequence from Schizosaccharomyces pombe 972h-. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 4.27Å
Experimental data:	Check
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ATD2_SCHPO ATPase histone chaperone which facilitates loading of histone H3-H4 onto DNA in an ATP-dependent manner (PubMed:31848341, PubMed:33658433). Plays a genome-wide role in nucleosome organization and establishment of chromatin (PubMed:26582768, PubMed:31848341). Also plays a role in heterochromatin assembly by stabilizing recruitment of the histone methyltransferase clr4 to methylated histone H3, to promote the transition from H3K9me2 to H3K9me3 (PubMed:32269268).^[1] ^[2] ^[3] ^[4]

References

↑ Gal C, Murton HE, Subramanian L, Whale AJ, Moore KM, Paszkiewicz K, Codlin S, Bähler J, Creamer KM, Partridge JF, Allshire RC, Kent NA, Whitehall SK. Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome occupancy and organisation. EMBO Rep. 2016 Jan;17(1):79-93. PMID:26582768 doi:10.15252/embr.201540476
↑ Cho C, Jang J, Kang Y, Watanabe H, Uchihashi T, Kim SJ, Kato K, Lee JY, Song JJ. Structural basis of nucleosome assembly by the Abo1 AAA+ ATPase histone chaperone. Nat Commun. 2019 Dec 17;10(1):5764. doi: 10.1038/s41467-019-13743-9. PMID:31848341 doi:http://dx.doi.org/10.1038/s41467-019-13743-9
↑ Dong W, Oya E, Zahedi Y, Prasad P, Svensson JP, Lennartsson A, Ekwall K, Durand-Dubief M. Abo1 is required for the H3K9me2 to H3K9me3 transition in heterochromatin. Sci Rep. 2020 Apr 8;10(1):6055. PMID:32269268 doi:10.1038/s41598-020-63209-y
↑ Kang Y, Cho C, Lee KS, Song JJ, Lee JY. Single-Molecule Imaging Reveals the Mechanism Underlying Histone Loading of Schizosaccharomyces pombe AAA+ ATPase Abo1. Mol Cells. 2021 Feb 28;44(2):79-87. PMID:33658433 doi:10.14348/molcells.2021.2242

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OCA

[1] Gal C, Murton HE, Subramanian L, Whale AJ, Moore KM, Paszkiewicz K, Codlin S, Bähler J, Creamer KM, Partridge JF, Allshire RC, Kent NA, Whitehall SK. Abo1, a conserved bromodomain AAA-ATPase, maintains global nucleosome occupancy and organisation. EMBO Rep. 2016 Jan;17(1):79-93. PMID:26582768 doi:10.15252/embr.201540476

[2] Cho C, Jang J, Kang Y, Watanabe H, Uchihashi T, Kim SJ, Kato K, Lee JY, Song JJ. Structural basis of nucleosome assembly by the Abo1 AAA+ ATPase histone chaperone. Nat Commun. 2019 Dec 17;10(1):5764. doi: 10.1038/s41467-019-13743-9. PMID:31848341 doi:http://dx.doi.org/10.1038/s41467-019-13743-9

[3] Dong W, Oya E, Zahedi Y, Prasad P, Svensson JP, Lennartsson A, Ekwall K, Durand-Dubief M. Abo1 is required for the H3K9me2 to H3K9me3 transition in heterochromatin. Sci Rep. 2020 Apr 8;10(1):6055. PMID:32269268 doi:10.1038/s41598-020-63209-y

[4] Kang Y, Cho C, Lee KS, Song JJ, Lee JY. Single-Molecule Imaging Reveals the Mechanism Underlying Histone Loading of Schizosaccharomyces pombe AAA+ ATPase Abo1. Mol Cells. 2021 Feb 28;44(2):79-87. PMID:33658433 doi:10.14348/molcells.2021.2242

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