6hkw

Crystal structure of human SDS22Crystal structure of human SDS22

Structural highlights

6hkw is a 5 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	PPP1R7, SDS22 (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PP1R7_HUMAN] Regulatory subunit of protein phosphatase 1.

Publication Abstract from PubMed

SDS22 is an ancient regulator of protein phosphatase-1 (PP1). Our crystal structure of SDS22 shows that its twelve leucine-rich repeats adopt a banana-shaped fold that is shielded from solvent by capping domains at its extremities. Subsequent modeling and biochemical studies revealed that the concave side of SDS22 likely interacts with PP1 helices alpha5 and alpha6, which are distal from the binding sites of many previously described PP1 interactors. Accordingly, we found that SDS22 acts as a "third" subunit of multiple PP1 holoenzymes. The crystal structure of SDS22 also revealed a large basic surface patch that enables binding of a phosphorylated form of splicing factor BCLAF1. Taken together, our data provide insights into the formation of PP1:SDS22 and the recruitment of additional interaction proteins, such as BCLAF1.

Structure-Guided Exploration of SDS22 Interactions with Protein Phosphatase PP1 and the Splicing Factor BCLAF1.,Heroes E, Van der Hoeven G, Choy MS, Garcia JDP, Ferreira M, Nys M, Derua R, Beullens M, Ulens C, Peti W, Van Meervelt L, Page R, Bollen M Structure. 2018 Dec 17. pii: S0969-2126(18)30458-1. doi:, 10.1016/j.str.2018.12.002. PMID:30661852^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Heroes E, Van der Hoeven G, Choy MS, Garcia JDP, Ferreira M, Nys M, Derua R, Beullens M, Ulens C, Peti W, Van Meervelt L, Page R, Bollen M. Structure-Guided Exploration of SDS22 Interactions with Protein Phosphatase PP1 and the Splicing Factor BCLAF1. Structure. 2018 Dec 17. pii: S0969-2126(18)30458-1. doi:, 10.1016/j.str.2018.12.002. PMID:30661852 doi:http://dx.doi.org/10.1016/j.str.2018.12.002

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OCA

[1] Heroes E, Van der Hoeven G, Choy MS, Garcia JDP, Ferreira M, Nys M, Derua R, Beullens M, Ulens C, Peti W, Van Meervelt L, Page R, Bollen M. Structure-Guided Exploration of SDS22 Interactions with Protein Phosphatase PP1 and the Splicing Factor BCLAF1. Structure. 2018 Dec 17. pii: S0969-2126(18)30458-1. doi:, 10.1016/j.str.2018.12.002. PMID:30661852 doi:http://dx.doi.org/10.1016/j.str.2018.12.002

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