6dxo

1.8 A structure of RsbN-BldN complex.1.8 A structure of RsbN-BldN complex.

Structural highlights 6dxo is a 2 chain structure with sequence from Streptomyces venezuelae ATCC 10712. This structure supersedes the now removed PDB entry 6c03. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function F2R911_STRVP Publication Abstract from PubMed Streptomyces are filamentous bacteria with a complex developmental life cycle characterized by the formation of spore-forming aerial hyphae. Transcription of the chaplin and rodlin genes, which are essential for aerial hyphae production, is directed by the extracytoplasmic function (ECF) sigma factor BldN, which is in turn controlled by an anti-sigma factor, RsbN. RsbN shows no sequence similarity to known anti-sigma factors and binds and inhibits BldN in an unknown manner. Here we describe the 2.23 A structure of the RsbN-BldN complex. The structure shows that BldN harbors sigma2 and sigma4 domains that are individually similar to other ECF sigma domains, which bind -10 and -35 promoter regions, respectively. The anti-sigma RsbN consists of three helices, with alpha3 forming a long helix embraced between BldN sigma2 and sigma4 while RsbN alpha1-alpha2 dock against sigma4 in a manner that would block -35 DNA binding. RsbN binding also freezes BldN in a conformation inactive for simultaneous -10 and -35 promoter interaction and RNAP binding. Strikingly, RsbN is structurally distinct from previously solved anti-sigma proteins. Thus, these data characterize the molecular determinants controlling a central Streptomyces developmental switch and reveal RsbN to be the founding member of a new structural class of anti-sigma factor. The crystal structure of the RsbN-sigmaBldN complex from Streptomyces venezuelae defines a new structural class of anti-sigma factor.,Schumacher MA, Bush MJ, Bibb MJ, Ramos-Leon F, Chandra G, Zeng W, Buttner MJ Nucleic Acids Res. 2018 Jun 14. pii: 5037724. doi: 10.1093/nar/gky493. PMID:29905823 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ectonucleotide pyrophosphatase/phosphodiesterase 3D structures Sigma factor 3D structures

spinqualitylabelsall models 6dxo, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image