5yio

NMR solution structure of subunit epsilon of the Mycobacterium tuberculosis F-ATP synthaseNMR solution structure of subunit epsilon of the Mycobacterium tuberculosis F-ATP synthase

Structural highlights 5yio is a 1 chain structure with sequence from Mycobacterium tuberculosis H37Rv. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ATPE_MYCTU Produces ATP from ADP in the presence of a proton gradient across the membrane. Publication Abstract from PubMed Mycobacterium tuberculosis (Mt) F1 F0 ATP synthase (alpha3 :beta3 :gamma:delta:epsilon:a:b:b':c9 ) is essential for the viability of growing and nongrowing persister cells of the pathogen. Here, we present the first NMR solution structure of Mtepsilon, revealing an N-terminal beta-barrel domain (NTD) and a C-terminal domain (CTD) composed of a helix-loop-helix with helix 1 and -2 being shorter compared to their counterparts in other bacteria. The C-terminal amino acids are oriented toward the NTD, forming a domain-domain interface between the NTD and CTD. The Mtepsilon structure provides a novel mechanistic model of coupling c-ring- and epsilon rotation via a patch of hydrophobic residues in the NTD and residues of the CTD to the bottom of the catalytic alpha3 beta3 -headpiece. To test our model, genome site-directed mutagenesis was employed to introduce amino acid changes in these two parts of the epsilon subunit. Inverted vesicle assays show that these mutations caused an increase in ATP hydrolysis activity and a reduction in ATP synthesis. The structural and enzymatic data are discussed in light of the transition mechanism of a compact and extended state of Mtepsilon, which provides the inhibitory effects of this coupling subunit inside the rotary engine. Finally, the employment of these data with molecular docking shed light into the second binding site of the drug Bedaquiline. DATABASE: Structural data are available in the PDB under the accession number 5YIO. The NMR solution structure of Mycobacterium tuberculosis F-ATP synthase subunit epsilon provides new insight into energy coupling inside the rotary engine.,Joon S, Ragunathan P, Sundararaman L, Nartey W, Kundu S, Manimekalai MSS, Bogdanovic N, Dick T, Gruber G FEBS J. 2018 Mar;285(6):1111-1128. doi: 10.1111/febs.14392. Epub 2018 Feb 6. PMID:29360236 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also ATPase 3D structures

spinqualitylabelsall models 5yio Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image