Lysozyme structure collected with 3D printed polymer mountsLysozyme structure collected with 3D printed polymer mounts

Structural highlights

5tk0 is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.795Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1]

Publication Abstract from PubMed

3D printed micrometer-scale polymer mounts for single crystal analysis have been prepared by photopolymerization using digital light projection stereolithography (DLP-SLA), with a commercially available digital light projection stereolithography printer (US$4000) and 3DM-ABS resin (US$150 per liter). The polymer mounts were prepared in batches of 49 in 1 h 15 min, which allowed for rapid prototyping and testing of new crystal mounting designs, with a resin cost of 0.2 cent US per mount. The suitability of the 3D printed mounts for single crystal crystallography has been demonstrated through their use in Cu Kalpha X-ray diffraction experiments of Rochelle salt (sodium potassium tartrate), the protein lysozyme, and has been employed for routine crystallographic analysis of organic and inorganic materials.

3D Printed Micrometer-Scale Polymer Mounts for Single Crystal Analysis.,Macdonald NP, Bunton GL, Park AY, Breadmore MC, Kilah NL Anal Chem. 2017 Mar 30. doi: 10.1021/acs.analchem.7b00443. PMID:28319372[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
  2. Macdonald NP, Bunton GL, Park AY, Breadmore MC, Kilah NL. 3D Printed Micrometer-Scale Polymer Mounts for Single Crystal Analysis. Anal Chem. 2017 Mar 30. doi: 10.1021/acs.analchem.7b00443. PMID:28319372 doi:http://dx.doi.org/10.1021/acs.analchem.7b00443

5tk0, resolution 1.79Å

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