5huq

A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemaseA tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase

Structural highlights

5huq is a 2 chain structure with sequence from Lactiplantibacillus plantarum. This structure supersedes the now removed PDB entry 4yns. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LARA_LACPL Catalyzes the interconversion between the D- and L-isomers of lactate (PubMed:24710389, PubMed:26138974). May act as a rescue enzyme to ensure D-lactate production in physiological conditions where its production by the D-lactate dehydrogenase LdhD is not sufficient (PubMed:16166538). D-Lactate is absolutely required for growth of L.plantarum and is an essential component of the cell wall peptidoglycan in this species, where it is incorporated as the last residue of the muramoyl-pentadepsipeptide peptidoglycan precursor; its incorporation confers high level of vancomycin resistance (PubMed:16166538).^[1] ^[2] ^[3]

References

↑ Goffin P, Deghorain M, Mainardi JL, Tytgat I, Champomier-Verges MC, Kleerebezem M, Hols P. Lactate racemization as a rescue pathway for supplying D-lactate to the cell wall biosynthesis machinery in Lactobacillus plantarum. J Bacteriol. 2005 Oct;187(19):6750-61. doi: 10.1128/JB.187.19.6750-6761.2005. PMID:16166538 doi:http://dx.doi.org/10.1128/JB.187.19.6750-6761.2005
↑ Desguin B, Goffin P, Viaene E, Kleerebezem M, Martin-Diaconescu V, Maroney MJ, Declercq JP, Soumillion P, Hols P. Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system. Nat Commun. 2014 Apr 7;5:3615. doi: 10.1038/ncomms4615. PMID:24710389 doi:http://dx.doi.org/10.1038/ncomms4615
↑ Desguin B, Zhang T, Soumillion P, Hols P, Hu J, Hausinger RP. METALLOPROTEINS. A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase. Science. 2015 Jul 3;349(6243):66-9. doi: 10.1126/science.aab2272. PMID:26138974 doi:http://dx.doi.org/10.1126/science.aab2272

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OCA

[1] Goffin P, Deghorain M, Mainardi JL, Tytgat I, Champomier-Verges MC, Kleerebezem M, Hols P. Lactate racemization as a rescue pathway for supplying D-lactate to the cell wall biosynthesis machinery in Lactobacillus plantarum. J Bacteriol. 2005 Oct;187(19):6750-61. doi: 10.1128/JB.187.19.6750-6761.2005. PMID:16166538 doi:http://dx.doi.org/10.1128/JB.187.19.6750-6761.2005

[2] Desguin B, Goffin P, Viaene E, Kleerebezem M, Martin-Diaconescu V, Maroney MJ, Declercq JP, Soumillion P, Hols P. Lactate racemase is a nickel-dependent enzyme activated by a widespread maturation system. Nat Commun. 2014 Apr 7;5:3615. doi: 10.1038/ncomms4615. PMID:24710389 doi:http://dx.doi.org/10.1038/ncomms4615

[3] Desguin B, Zhang T, Soumillion P, Hols P, Hu J, Hausinger RP. METALLOPROTEINS. A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase. Science. 2015 Jul 3;349(6243):66-9. doi: 10.1126/science.aab2272. PMID:26138974 doi:http://dx.doi.org/10.1126/science.aab2272

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