5gsm

Glycoside hydrolase B with productGlycoside hydrolase B with product

Structural highlights

5gsm is a 2 chain structure with sequence from Thermococcus kodakarensis KOD1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.27Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GLMA_THEKO Exo-type enzyme that specifically cleaves the non-reducing terminal glycosidic bond of chitooligosaccharides (PubMed:12923090, PubMed:15136574, PubMed:28130448). Catalyzes the hydrolysis of GlcN-GlcNAc to glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) (PubMed:15136574, PubMed:28130448). Involved in chitin degradation (PubMed:12923090, PubMed:15136574). Can also hydrolyze reduced chitobiose (GlcN2OH) and chitooligosaccharides of various chain lengths (PubMed:12923090).^[1] ^[2] ^[3]

References

↑ Tanaka T, Fukui T, Atomi H, Imanaka T. Characterization of an exo-beta-D-glucosaminidase involved in a novel chitinolytic pathway from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J Bacteriol. 2003 Sep;185(17):5175-81. PMID:12923090 doi:10.1128/JB.185.17.5175-5181.2003
↑ Tanaka T, Fukui T, Fujiwara S, Atomi H, Imanaka T. Concerted action of diacetylchitobiose deacetylase and exo-beta-D-glucosaminidase in a novel chitinolytic pathway in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J Biol Chem. 2004 Jul 16;279(29):30021-7. PMID:15136574 doi:10.1074/jbc.M314187200
↑ Mine S, Watanabe M, Kamachi S, Abe Y, Ueda T. The Structure of an Archaeal β-Glucosaminidase Provides Insight into Glycoside Hydrolase Evolution. J Biol Chem. 2017 Mar 24;292(12):4996-5006. PMID:28130448 doi:10.1074/jbc.M116.766535

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OCA

[1] Tanaka T, Fukui T, Atomi H, Imanaka T. Characterization of an exo-beta-D-glucosaminidase involved in a novel chitinolytic pathway from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J Bacteriol. 2003 Sep;185(17):5175-81. PMID:12923090 doi:10.1128/JB.185.17.5175-5181.2003

[2] Tanaka T, Fukui T, Fujiwara S, Atomi H, Imanaka T. Concerted action of diacetylchitobiose deacetylase and exo-beta-D-glucosaminidase in a novel chitinolytic pathway in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1. J Biol Chem. 2004 Jul 16;279(29):30021-7. PMID:15136574 doi:10.1074/jbc.M314187200

[3] Mine S, Watanabe M, Kamachi S, Abe Y, Ueda T. The Structure of an Archaeal β-Glucosaminidase Provides Insight into Glycoside Hydrolase Evolution. J Biol Chem. 2017 Mar 24;292(12):4996-5006. PMID:28130448 doi:10.1074/jbc.M116.766535

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