5d3m
Folate ECF transporter: AMPPNP bound stateFolate ECF transporter: AMPPNP bound state
Structural highlights
FunctionECFA1_LACDA ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates. Publication Abstract from PubMedEnergy-coupling factor (ECF) transporters mediate uptake of micronutrients in prokaryotes. The transporters consist of an S-component that binds the transported substrate and an ECF module (EcfAA'T) that binds and hydrolyses ATP. The mechanism of transport is poorly understood but presumably involves an unusual step in which the membrane-embedded S-component topples over to carry the substrate across the membrane. In many ECF transporters, the S-component dissociates from the ECF module after transport. Subsequently, substrate-bound S-components out-compete the empty proteins for re-binding to the ECF module in a new round of transport. Here we present crystal structures of the folate-specific transporter ECF-FolT from Lactobacillus delbrueckii. Interaction of the ECF module with FolT stabilizes the toppled state, and simultaneously destroys the high-affinity folate-binding site, allowing substrate release into the cytosol. We hypothesize that differences in the kinetics of toppling can explain how substrate-loaded FolT out-competes apo-FolT for association with the ECF module. Structural insight in the toppling mechanism of an energy-coupling factor transporter.,Swier LJ, Guskov A, Slotboom DJ Nat Commun. 2016 Mar 30;7:11072. doi: 10.1038/ncomms11072. PMID:27026363[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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