5cm9
Structural Basis for the Selectivity of Guanine Nucleotide Exchange Factors for the small G-protein RalStructural Basis for the Selectivity of Guanine Nucleotide Exchange Factors for the small G-protein Ral
Structural highlights
FunctionRGL2_MOUSE Probable guanine nucleotide exchange factor. Putative effector of Ras and/or Rap. Associates with the GTP-bound form of Rap 1A and H-Ras in vitro. Publication Abstract from PubMedCDC25 homology domain (CDC25-HD) containing Guanine Nucleotide Exchange Factors (GEFs) initiate signalling by small G-proteins of the Ras-family. Each GEF acts on a small subset of the G-proteins only, thus providing signalling selectivity. Rlf is a GEF with selectivity for the G-proteins RalA and RalB. Here the crystal structure of Rlf in complex with Ral is determined. The Rlf.Ral complex crystallised into two different crystal forms, which represent different steps of the exchange reaction. Thereby general insight in the CDC25-HD catalysed nucleotide exchange is obtained. In addition, the basis for the selectivity of the interaction is investigated. The exchange activity is monitored by the use of recombinant proteins. Selectivity determinants in the binding interface are identified and confirmed by a mutational study. The structure of the Guanine Nucleotide Exchange Factor Rlf in complex with the small G-protein Ral identifies conformational intermediates of the exchange reaction and the basis for the selectivity.,Popovic M, Schouten A, Rensen-de Leeuw M, Rehmann H J Struct Biol. 2015 Dec 11. pii: S1047-8477(15)30112-X. doi:, 10.1016/j.jsb.2015.12.006. PMID:26687416[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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