4wh4

Protein GB1 Quadruple Mutant I6H/N8H/K28H/Q32HProtein GB1 Quadruple Mutant I6H/N8H/K28H/Q32H

Structural highlights 4wh4 is a 2 chain structure with sequence from Streptococcus sp. 'group G'. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.2Å Ligands: , Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SPG2_STRSG Publication Abstract from PubMed The development of ESR methods that measure long-range distance distributions has advanced biophysical research. However, the spin labels commonly employed are highly flexible, which leads to ambiguity in relating ESR measurements to protein-backbone structure. Herein we present the double-histidine (dHis) Cu(2+)-binding motif as a rigid spin probe for double electron-electron resonance (DEER) distance measurements. The spin label is assembled in situ from natural amino acid residues and a metal salt, requires no postexpression synthetic modification, and provides distance distributions that are dramatically narrower than those found with the commonly used protein spin label. Simple molecular modeling based on an X-ray crystal structure of an unlabeled protein led to a predicted most probable distance within 0.5 A of the experimental value. Cu(2+) DEER with the dHis motif shows great promise for the resolution of precise, unambiguous distance constraints that relate directly to protein-backbone structure and flexibility. The double-histidine Cu(2)(+)-binding motif: a highly rigid, site-specific spin probe for electron spin resonance distance measurements.,Cunningham TF, Putterman MR, Desai A, Horne WS, Saxena S Angew Chem Int Ed Engl. 2015 May 18;54(21):6330-4. doi: 10.1002/anie.201501968., Epub 2015 Mar 27. PMID:25821033 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Protein G

spinqualitylabelsall models 4wh4, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image