Proteopedia

4uhv

The structure of VgrG1, the needle tip of the bacterial Type VI Secretion SystemThe structure of VgrG1, the needle tip of the bacterial Type VI Secretion System

Structural highlights

4uhv is a 2 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

VGR1A_PSEAE Part of the H1 type VI secretion system (H1-T6SS) specialized secretion system, which delivers several virulence factors in both prokaryotic and eukaryotic cells during infection (PubMed:21325275, PubMed:24794869). Forms the spike at the tip of the elongating tube formed by haemolysin co-regulated protein 1/Hcp1 (PubMed:26894531). Allows the delivery of the Tse6 toxin to target cells where it exerts its toxicity (PubMed:30177742).[1] [2] [3] [4]

Publication Abstract from PubMed

When 300 kV cryo-EM images at Scherzer focus are acquired from approximately 100 nm thick three-dimensional protein nanocrystals using a Falcon 2 direct electron detector, Fourier transformation can reveal the crystalline lattice to surprisingly high resolutions, even though the images themselves seem to be devoid of any contrast. Here, it is reported how this lattice information can be enhanced by means of a wave finder in combination with Wiener-type maximum-likelihood filtering. This procedure paves the way towards full three-dimensional structure determination at high resolution for protein crystals.

Lattice filter for processing image data of three-dimensional protein nanocrystals.,van Genderen E, Li YW, Nederlof I, Abrahams JP Acta Crystallogr D Struct Biol. 2016 Jan;72(Pt 1):34-9. doi:, 10.1107/S205979831502149X. Epub 2016 Jan 1. PMID:26894532[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Hachani A, Lossi NS, Hamilton A, Jones C, Bleves S, Albesa-Jove D, Filloux A. Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 2011 Apr 8;286(14):12317-27. doi: 10.1074/jbc.M110.193045. Epub 2011 , Feb 16. PMID:21325275 doi:http://dx.doi.org/10.1074/jbc.M110.193045
  2. ↑ Hachani A, Allsopp LP, Oduko Y, Filloux A. The VgrG proteins are "a la carte" delivery systems for bacterial type VI effectors. J Biol Chem. 2014 Jun 20;289(25):17872-84. doi: 10.1074/jbc.M114.563429. Epub , 2014 May 2. PMID:24794869 doi:http://dx.doi.org/10.1074/jbc.M114.563429
  3. ↑ Spinola-Amilibia M, Davo-Siguero I, Ruiz FM, Santillana E, Medrano FJ, Romero A. The structure of VgrG1 from Pseudomonas aeruginosa, the needle tip of the bacterial type VI secretion system. Acta Crystallogr D Struct Biol. 2016 Jan;72(Pt 1):22-33. doi: , 10.1107/S2059798315021142. Epub 2016 Jan 1. PMID:26894531 doi:http://dx.doi.org/10.1107/S2059798315021142
  4. ↑ Quentin D, Ahmad S, Shanthamoorthy P, Mougous JD, Whitney JC, Raunser S. Mechanism of loading and translocation of type VI secretion system effector Tse6. Nat Microbiol. 2018 Sep 3. pii: 10.1038/s41564-018-0238-z. doi:, 10.1038/s41564-018-0238-z. PMID:30177742 doi:http://dx.doi.org/10.1038/s41564-018-0238-z
  5. ↑ van Genderen E, Li YW, Nederlof I, Abrahams JP. Lattice filter for processing image data of three-dimensional protein nanocrystals. Acta Crystallogr D Struct Biol. 2016 Jan;72(Pt 1):34-9. doi:, 10.1107/S205979831502149X. Epub 2016 Jan 1. PMID:26894532 doi:http://dx.doi.org/10.1107/S205979831502149X

4uhv, resolution 2.00Å

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