4hsq

Crystal Structure of Domains D2 and D3 of the Major Pilin SpaD from Corynebacterium diphtheriaeCrystal Structure of Domains D2 and D3 of the Major Pilin SpaD from Corynebacterium diphtheriae

Structural highlights 4hsq is a 1 chain structure with sequence from Corynebacterium diphtheriae NCTC 13129. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.87Å Ligands: , Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q6NK05_CORDI Publication Abstract from PubMed The Gram-positive organism Corynebacterium diphtheriae, the cause of diphtheria in humans, expresses pili on its surface which it uses for adhesion and colonization of its host. These pili are covalent protein polymers composed of three types of pilin subunit that are assembled by specific sortase enzymes. A structural analysis of the major pilin SpaD, which forms the polymeric backbone of one of the three types of pilus expressed by C. diphtheriae, is reported. Mass-spectral and crystallographic analysis shows that SpaD contains three internal Lys-Asn isopeptide bonds. One of these, shown by mass spectrometry to be located in the N-terminal D1 domain of the protein, only forms slowly, implying an energy barrier to bond formation. Two crystal structures, of the full-length three-domain protein at 2.5 A resolution and of a two-domain (D2-D3) construct at 1.87 A resolution, show that each of the three Ig-like domains contains a single Lys-Asn isopeptide-bond cross-link, assumed to give mechanical stability as in other such pili. Additional stabilizing features include a disulfide bond in the D3 domain and a calcium-binding loop in D2. The N-terminal D1 domain is more flexible than the others and, by analogy with other major pilins of this type, the slow formation of its isopeptide bond can be attributed to its location adjacent to the lysine used in sortase-mediated polymerization during pilus assembly. A slow-forming isopeptide bond in the structure of the major pilin SpaD from Corynebacterium diphtheriae has implications for pilus assembly.,Kang HJ, Paterson NG, Kim CU, Middleditch M, Chang C, Ton-That H, Baker EN Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1190-201. doi:, 10.1107/S1399004714001400. Epub 2014 Apr 26. PMID:24816089 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Pilin 3D structures

spinqualitylabelsall models 4hsq, resolution 1.87Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image