4fx9

Structure of the Pyrococcus horikoshii CoA persulfide/polysulfide reductaseStructure of the Pyrococcus horikoshii CoA persulfide/polysulfide reductase

Structural highlights

4fx9 is a 2 chain structure with sequence from Pyrococcus horikoshii OT3. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NCPPR_PYRHO Catalyzes the NAD(P)H-dependent reduction of polysulfide, CoA-polysulfides, and CoA persulfide, as well as the reduction of a range of other small persulfides, including TNB and glutathione persulfides. The likely in vivo substrates are di-, poly-, and persulfide derivatives of coenzyme A, although polysulfide itself is also efficiently reduced (PubMed:23530771). Shows coenzyme A disulfide reductase (CoADR) activity with both NADH and NADPH, with a preference for NADPH (PubMed:15720393). May also play a role in the reduction of elemental sulfur (PubMed:23530771).^[1] ^[2]

References

↑ Harris DR, Ward DE, Feasel JM, Lancaster KM, Murphy RD, Mallet TC, Crane EJ 3rd. Discovery and characterization of a Coenzyme A disulfide reductase from Pyrococcus horikoshii. Implications for this disulfide metabolism of anaerobic hyperthermophiles. FEBS J. 2005 Mar;272(5):1189-200. PMID:15720393 doi:EJB4555
↑ Herwald S, Liu AY, Zhu BE, Sea KW, Lopez KM, Sazinsky MH, Crane Iii EJ. Characterization of the structure and substrate specificity of the pyrococcal CoADR/Psr: Implications for S0-based respiration and a sulfur-dependent antioxidant system in Pyrococcus. Biochemistry. 2013 Mar 26. PMID:23530771 doi:10.1021/bi3014399

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OCA

[1] Harris DR, Ward DE, Feasel JM, Lancaster KM, Murphy RD, Mallet TC, Crane EJ 3rd. Discovery and characterization of a Coenzyme A disulfide reductase from Pyrococcus horikoshii. Implications for this disulfide metabolism of anaerobic hyperthermophiles. FEBS J. 2005 Mar;272(5):1189-200. PMID:15720393 doi:EJB4555

[2] Herwald S, Liu AY, Zhu BE, Sea KW, Lopez KM, Sazinsky MH, Crane Iii EJ. Characterization of the structure and substrate specificity of the pyrococcal CoADR/Psr: Implications for S0-based respiration and a sulfur-dependent antioxidant system in Pyrococcus. Biochemistry. 2013 Mar 26. PMID:23530771 doi:10.1021/bi3014399

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