4edn

Crystal structure of beta-parvin CH2 domain in complex with paxillin LD1 motifCrystal structure of beta-parvin CH2 domain in complex with paxillin LD1 motif

Structural highlights

4edn is a 17 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.9Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PARVB_HUMAN Adapter protein that plays a role in integrin signaling via ILK and in activation of the GTPases CDC42 and RAC1 by guanine exchange factors, such as ARHGEF6. Is involved in the reorganization of the actin cytoskeleton and formation of lamellipodia. Plays a role in cell adhesion, cell spreading, establishment or maintenance of cell polarity, and cell migration.^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

beta-Parvin is a cytoplasmic adaptor protein that localizes to focal adhesions where it interacts with integrin-linked kinase and is involved in linking integrin receptors to the cytoskeleton. It has been reported that despite high sequence similarity to alpha-parvin, beta-parvin does not bind paxillin, suggesting distinct interactions and cellular functions for these two closely related parvins. Here, we reveal that beta-parvin binds directly and specifically to leucine-aspartic acid repeat (LD) motifs in paxillin via its C-terminal calponin homology (CH2) domain. We present the co-crystal structure of beta-parvin CH2 domain in complex with paxillin LD1 motif to 2.9 A resolution and find that the interaction is similar to that previously observed between alpha-parvin and paxillin LD1. We also present crystal structures of unbound beta-parvin CH2 domain at 2.1 A and 2.0 A resolution that show significant conformational flexibility in the N-terminal alpha-helix, suggesting an induced fit upon paxillin binding. We find that beta-parvin has specificity for the LD1, LD2, and LD4 motifs of paxillin, with K(D) values determined to 27, 42, and 73 mum, respectively, by surface plasmon resonance. Furthermore, we show that proper localization of beta-parvin to focal adhesions requires both the paxillin and integrin-linked kinase binding sites and that paxillin is important for early targeting of beta-parvin. These studies provide the first molecular details of beta-parvin binding to paxillin and help define the requirements for beta-parvin localization to focal adhesions.

Structural Basis for Paxillin Binding and Focal Adhesion Targeting of beta-Parvin.,Stiegler AL, Draheim KM, Li X, Chayen NE, Calderwood DA, Boggon TJ J Biol Chem. 2012 Sep 21;287(39):32566-77. Epub 2012 Aug 6. PMID:22869380^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Yamaji S, Suzuki A, Sugiyama Y, Koide Y, Yoshida M, Kanamori H, Mohri H, Ohno S, Ishigatsubo Y. A novel integrin-linked kinase-binding protein, affixin, is involved in the early stage of cell-substrate interaction. J Cell Biol. 2001 Jun 11;153(6):1251-64. PMID:11402068
↑ Mishima W, Suzuki A, Yamaji S, Yoshimi R, Ueda A, Kaneko T, Tanaka J, Miwa Y, Ohno S, Ishigatsubo Y. The first CH domain of affixin activates Cdc42 and Rac1 through alphaPIX, a Cdc42/Rac1-specific guanine nucleotide exchanging factor. Genes Cells. 2004 Mar;9(3):193-204. PMID:15005707
↑ Zhang Y, Chen K, Tu Y, Wu C. Distinct roles of two structurally closely related focal adhesion proteins, alpha-parvins and beta-parvins, in regulation of cell morphology and survival. J Biol Chem. 2004 Oct 1;279(40):41695-705. Epub 2004 Jul 28. PMID:15284246 doi:10.1074/jbc.M401563200
↑ Yamaji S, Suzuki A, Kanamori H, Mishima W, Yoshimi R, Takasaki H, Takabayashi M, Fujimaki K, Fujisawa S, Ohno S, Ishigatsubo Y. Affixin interacts with alpha-actinin and mediates integrin signaling for reorganization of F-actin induced by initial cell-substrate interaction. J Cell Biol. 2004 May 24;165(4):539-51. PMID:15159419 doi:10.1083/jcb.200308141
↑ Matsuda C, Kameyama K, Suzuki A, Mishima W, Yamaji S, Okamoto H, Nishino I, Hayashi YK. Affixin activates Rac1 via betaPIX in C2C12 myoblast. FEBS Lett. 2008 Apr 9;582(8):1189-96. doi: 10.1016/j.febslet.2008.01.064. Epub, 2008 Mar 4. PMID:18325335 doi:10.1016/j.febslet.2008.01.064
↑ Stiegler AL, Draheim KM, Li X, Chayen NE, Calderwood DA, Boggon TJ. Structural Basis for Paxillin Binding and Focal Adhesion Targeting of beta-Parvin. J Biol Chem. 2012 Sep 21;287(39):32566-77. Epub 2012 Aug 6. PMID:22869380 doi:10.1074/jbc.M112.367342

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Yamaji S, Suzuki A, Sugiyama Y, Koide Y, Yoshida M, Kanamori H, Mohri H, Ohno S, Ishigatsubo Y. A novel integrin-linked kinase-binding protein, affixin, is involved in the early stage of cell-substrate interaction. J Cell Biol. 2001 Jun 11;153(6):1251-64. PMID:11402068

[2] Mishima W, Suzuki A, Yamaji S, Yoshimi R, Ueda A, Kaneko T, Tanaka J, Miwa Y, Ohno S, Ishigatsubo Y. The first CH domain of affixin activates Cdc42 and Rac1 through alphaPIX, a Cdc42/Rac1-specific guanine nucleotide exchanging factor. Genes Cells. 2004 Mar;9(3):193-204. PMID:15005707

[3] Zhang Y, Chen K, Tu Y, Wu C. Distinct roles of two structurally closely related focal adhesion proteins, alpha-parvins and beta-parvins, in regulation of cell morphology and survival. J Biol Chem. 2004 Oct 1;279(40):41695-705. Epub 2004 Jul 28. PMID:15284246 doi:10.1074/jbc.M401563200

[4] Yamaji S, Suzuki A, Kanamori H, Mishima W, Yoshimi R, Takasaki H, Takabayashi M, Fujimaki K, Fujisawa S, Ohno S, Ishigatsubo Y. Affixin interacts with alpha-actinin and mediates integrin signaling for reorganization of F-actin induced by initial cell-substrate interaction. J Cell Biol. 2004 May 24;165(4):539-51. PMID:15159419 doi:10.1083/jcb.200308141

[5] Matsuda C, Kameyama K, Suzuki A, Mishima W, Yamaji S, Okamoto H, Nishino I, Hayashi YK. Affixin activates Rac1 via betaPIX in C2C12 myoblast. FEBS Lett. 2008 Apr 9;582(8):1189-96. doi: 10.1016/j.febslet.2008.01.064. Epub, 2008 Mar 4. PMID:18325335 doi:10.1016/j.febslet.2008.01.064

[6] Stiegler AL, Draheim KM, Li X, Chayen NE, Calderwood DA, Boggon TJ. Structural Basis for Paxillin Binding and Focal Adhesion Targeting of beta-Parvin. J Biol Chem. 2012 Sep 21;287(39):32566-77. Epub 2012 Aug 6. PMID:22869380 doi:10.1074/jbc.M112.367342

[1]

[2]

[3]

[4]

[5]

[6]