4aco
Structure of the budding yeast Ndc10 N-terminal domainStructure of the budding yeast Ndc10 N-terminal domain
Structural highlights
FunctionCBF3A_YEAST Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. Publication Abstract from PubMedWe have solved the X-ray structure of the amino-terminal half of the yeast kinetochore protein Ndc10 at 1.8 Angstrom resolution. This essential protein is a key constituent of the budding yeast centromere, and is essential for the recruitment of the centromeric nucleosome and establishment of the kinetochore. The fold of the protein shows unexpected similarities to the tyrosine recombinase/lambda-integrase family of proteins, most notably Cre, with some variation in the relative position of the sub-domains. This finding offers new insights into kinetochore evolution and the adaptation of a well-studied protein fold to a novel role. By comparison to tyrosine recombinases, and mutagenesis studies, we have been able to define some of the key DNA-binding motifs. The structure of the yeast kinetochore Ndc10 DNA-binding domain reveals an unexpected evolutionary relationship to tyrosine recombinases.,Perriches T, Singleton MR J Biol Chem. 2012 Jan 3. PMID:22215672[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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