3wmu

The structure of an anti-cancer lectin mytilec apo-form from the mussel Mytilus galloprovincialisThe structure of an anti-cancer lectin mytilec apo-form from the mussel Mytilus galloprovincialis

Structural highlights

3wmu is a 2 chain structure with sequence from Mytilus galloprovincialis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.1Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LEC1_MYTGA Alpha-D-galactose-binding lectin (PubMed:27187419, PubMed:23093409, PubMed:27321048). Binds D-GalNAc, but not glucose or its derivatives (PubMed:27321048). Has hemagglutinating activity towards rabbit erythrocytes (PubMed:23093409, PubMed:27321048). Agglutinates bacteria. Has bacteriostatic activity on both Gram-positive and Gram-negative bacteria including B.subtilis, S.aureus, E.coli and V.parahaemolyticus, respectively (PubMed:27187419). Has a dose-dependent cytotoxic effect on the human globotriaosylceramide (Gb3)-expressing Epstein-Barr virus (EBV)-positive Burkitt's lymphoma (Raji) cell line (PubMed:23093409, PubMed:27321048). Has dose-dependent cytotoxic effect on another Burkitt's lymphoma (Ramos) cell line, which does not possess the EBV genome, but also expresses Gb3. Binds to Gb3 in these cells leading to phosphorylation of MEK1/2, ERK1/2, JNK and p38 kinase, activation of caspase-9/3 and to expression of p21 and tumor necrosis factor (TNF)-alpha (PubMed:26694420). No cytotoxic effect on the human chronic myelogenous leukemia (K-562) cell line, which does not express Gb3 (PubMed:23093409, PubMed:26694420). May be involved in innate immunity acting as an antibacterial or antifungal agent (PubMed:26694420, PubMed:27321048). May be a pattern recognition receptor (PRR) involved in recognition of glycans found on parasitic or symbiotic microorganisms (PubMed:27187419).^[1] ^[2] ^[3] ^[4]

References

↑ Fujii Y, Dohmae N, Takio K, Kawsar SM, Matsumoto R, Hasan I, Koide Y, Kanaly RA, Yasumitsu H, Ogawa Y, Sugawara S, Hosono M, Nitta K, Hamako J, Matsui T, Ozeki Y. A lectin from the mussel Mytilus galloprovincialis has a highly novel primary structure and induces glycan-mediated cytotoxicity of globotriaosylceramide-expressing lymphoma cells. J Biol Chem. 2012 Dec 28;287(53):44772-83. PMID:23093409 doi:10.1074/jbc.M112.418012
↑ Hasan I, Sugawara S, Fujii Y, Koide Y, Terada D, Iimura N, Fujiwara T, Takahashi KG, Kojima N, Rajia S, Kawsar SM, Kanaly RA, Uchiyama H, Hosono M, Ogawa Y, Fujita H, Hamako J, Matsui T, Ozeki Y. MytiLec, a Mussel R-Type Lectin, Interacts with Surface Glycan Gb3 on Burkitt's Lymphoma Cells to Trigger Apoptosis through Multiple Pathways. Mar Drugs. 2015 Dec 14;13(12):7377-89. PMID:26694420 doi:10.3390/md13127071
↑ Hasan I, Gerdol M, Fujii Y, Rajia S, Koide Y, Yamamoto D, Kawsar SM, Ozeki Y. cDNA and Gene Structure of MytiLec-1, A Bacteriostatic R-Type Lectin from the Mediterranean Mussel (Mytilus galloprovincialis). Mar Drugs. 2016 May 11;14(5):92. PMID:27187419 doi:10.3390/md14050092
↑ Terada D, Kawai F, Noguchi H, Unzai S, Hasan I, Fujii Y, Park SY, Ozeki Y, Tame JR. Crystal structure of MytiLec, a galactose-binding lectin from the mussel Mytilus galloprovincialis with cytotoxicity against certain cancer cell types. Sci Rep. 2016 Jun 20;6:28344. doi: 10.1038/srep28344. PMID:27321048 doi:http://dx.doi.org/10.1038/srep28344

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OCA

[1] Fujii Y, Dohmae N, Takio K, Kawsar SM, Matsumoto R, Hasan I, Koide Y, Kanaly RA, Yasumitsu H, Ogawa Y, Sugawara S, Hosono M, Nitta K, Hamako J, Matsui T, Ozeki Y. A lectin from the mussel Mytilus galloprovincialis has a highly novel primary structure and induces glycan-mediated cytotoxicity of globotriaosylceramide-expressing lymphoma cells. J Biol Chem. 2012 Dec 28;287(53):44772-83. PMID:23093409 doi:10.1074/jbc.M112.418012

[2] Hasan I, Sugawara S, Fujii Y, Koide Y, Terada D, Iimura N, Fujiwara T, Takahashi KG, Kojima N, Rajia S, Kawsar SM, Kanaly RA, Uchiyama H, Hosono M, Ogawa Y, Fujita H, Hamako J, Matsui T, Ozeki Y. MytiLec, a Mussel R-Type Lectin, Interacts with Surface Glycan Gb3 on Burkitt's Lymphoma Cells to Trigger Apoptosis through Multiple Pathways. Mar Drugs. 2015 Dec 14;13(12):7377-89. PMID:26694420 doi:10.3390/md13127071

[3] Hasan I, Gerdol M, Fujii Y, Rajia S, Koide Y, Yamamoto D, Kawsar SM, Ozeki Y. cDNA and Gene Structure of MytiLec-1, A Bacteriostatic R-Type Lectin from the Mediterranean Mussel (Mytilus galloprovincialis). Mar Drugs. 2016 May 11;14(5):92. PMID:27187419 doi:10.3390/md14050092

[4] Terada D, Kawai F, Noguchi H, Unzai S, Hasan I, Fujii Y, Park SY, Ozeki Y, Tame JR. Crystal structure of MytiLec, a galactose-binding lectin from the mussel Mytilus galloprovincialis with cytotoxicity against certain cancer cell types. Sci Rep. 2016 Jun 20;6:28344. doi: 10.1038/srep28344. PMID:27321048 doi:http://dx.doi.org/10.1038/srep28344

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