3wfs

tRNA processing enzyme complex 3tRNA processing enzyme complex 3

Structural highlights

3wfs is a 4 chain structure with sequence from Aquifex aeolicus VF5, Thermotoga maritima MSB8 and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.311Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CATNT_AQUAE tRNA nucleotidyltransferase involved in the synthesis of the tRNA CCA terminus. Adds the two cytidine residues to tRNA.^[1] ^[2]

Publication Abstract from PubMed

The 3'-terminal CCA (CCA-3' at positions 74-76) of tRNA is synthesized by CCA-adding enzyme using CTP and ATP as substrates, without a nucleic acid template. In Aquifex aeolicus, CC-adding and A-adding enzymes collaboratively synthesize the CCA-3'. The mechanism of CCA-3' synthesis by these two enzymes remained obscure. We now present crystal structures representing CC addition onto tRNA by A. aeolicus CC-adding enzyme. After C(7)(4) addition in an enclosed active pocket and pyrophosphate release, the tRNA translocates and rotates relative to the enzyme, and C(7)(5) addition occurs in the same active pocket as C(7)(4) addition. At both the C(7)(4)-adding and C(7)(5)-adding stages, CTP is selected by Watson-Crick-like hydrogen bonds between the cytosine of CTP and conserved Asp and Arg residues in the pocket. After C(7)(4)C(7)(5) addition and pyrophosphate release, the tRNA translocates further and drops off the enzyme, and the CC-adding enzyme terminates RNA polymerization.

Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase.,Yamashita S, Takeshita D, Tomita K Structure. 2014 Feb 4;22(2):315-25. doi: 10.1016/j.str.2013.12.002. Epub 2014 Jan, 2. PMID:24389024^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tomita K, Weiner AM. Collaboration between CC 3'-terminal CCA of tRNA in Aquifex aeolicus. Science. 2001 Nov 9;294(5545):1334-6. PMID:11701927 doi:10.1126/science.1063816
↑ Yamashita S, Takeshita D, Tomita K. Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase. Structure. 2014 Feb 4;22(2):315-25. doi: 10.1016/j.str.2013.12.002. Epub 2014 Jan, 2. PMID:24389024 doi:http://dx.doi.org/10.1016/j.str.2013.12.002
↑ Yamashita S, Takeshita D, Tomita K. Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase. Structure. 2014 Feb 4;22(2):315-25. doi: 10.1016/j.str.2013.12.002. Epub 2014 Jan, 2. PMID:24389024 doi:http://dx.doi.org/10.1016/j.str.2013.12.002

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OCA

[1] Tomita K, Weiner AM. Collaboration between CC 3'-terminal CCA of tRNA in Aquifex aeolicus. Science. 2001 Nov 9;294(5545):1334-6. PMID:11701927 doi:10.1126/science.1063816

[2] Yamashita S, Takeshita D, Tomita K. Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase. Structure. 2014 Feb 4;22(2):315-25. doi: 10.1016/j.str.2013.12.002. Epub 2014 Jan, 2. PMID:24389024 doi:http://dx.doi.org/10.1016/j.str.2013.12.002

[3] Yamashita S, Takeshita D, Tomita K. Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase. Structure. 2014 Feb 4;22(2):315-25. doi: 10.1016/j.str.2013.12.002. Epub 2014 Jan, 2. PMID:24389024 doi:http://dx.doi.org/10.1016/j.str.2013.12.002

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