3wfa

Catalytic role of the calcium ion in GH97 inverting glycoside hydrolaseCatalytic role of the calcium ion in GH97 inverting glycoside hydrolase

Structural highlights

3wfa is a 2 chain structure with sequence from Bacteroides thetaiotaomicron. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SUSB_BACTN Glucoamylase that hydrolyzes alpha-1,4-glucosidic linkages, alpha-1,6-, alpha-1,3- and alpha-1,2-glucosidic linkages during starch degradation.^[1] ^[2]

Publication Abstract from PubMed

The role of calcium ion in the active site of the inverting glycoside hydrolase family 97 enzyme, BtGH97a, was investigated through structural and kinetic studies. The calcium ion was likely directly involved in the catalytic reaction. The pH dependence of kcat/Km values in the presence or absence of calcium ion indicated that the calcium ion lowered the pKa of the base catalyst. The significant decreases in kcat/Km for hydrolysis of substrates with basic leaving groups in the absence of calcium ion confirmed that the calcium ion facilitated the leaving group departure.

Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase.,Okuyama M, Yoshida T, Hondoh H, Mori H, Yao M, Kimura A FEBS Lett. 2014 Aug 25;588(17):3213-7. doi: 10.1016/j.febslet.2014.07.002. Epub , 2014 Jul 10. PMID:25017438^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ D'Elia JN, Salyers AA. Contribution of a neopullulanase, a pullulanase, and an alpha-glucosidase to growth of Bacteroides thetaiotaomicron on starch. J Bacteriol. 1996 Dec;178(24):7173-9. PMID:8955399
↑ Kitamura M, Okuyama M, Tanzawa F, Mori H, Kitago Y, Watanabe N, Kimura A, Tanaka I, Yao M. Structural and functional analysis of a glycoside hydrolase family 97 enzyme from Bacteroides thetaiotaomicron. J Biol Chem. 2008 Dec 26;283(52):36328-37. Epub 2008 Nov 3. PMID:18981178 doi:10.1074/jbc.M806115200
↑ Okuyama M, Yoshida T, Hondoh H, Mori H, Yao M, Kimura A. Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase. FEBS Lett. 2014 Aug 25;588(17):3213-7. PMID:25017438 doi:10.1016/j.febslet.2014.07.002

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OCA

[1] D'Elia JN, Salyers AA. Contribution of a neopullulanase, a pullulanase, and an alpha-glucosidase to growth of Bacteroides thetaiotaomicron on starch. J Bacteriol. 1996 Dec;178(24):7173-9. PMID:8955399

[2] Kitamura M, Okuyama M, Tanzawa F, Mori H, Kitago Y, Watanabe N, Kimura A, Tanaka I, Yao M. Structural and functional analysis of a glycoside hydrolase family 97 enzyme from Bacteroides thetaiotaomicron. J Biol Chem. 2008 Dec 26;283(52):36328-37. Epub 2008 Nov 3. PMID:18981178 doi:10.1074/jbc.M806115200

[3] Okuyama M, Yoshida T, Hondoh H, Mori H, Yao M, Kimura A. Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase. FEBS Lett. 2014 Aug 25;588(17):3213-7. PMID:25017438 doi:10.1016/j.febslet.2014.07.002

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