3rfv

Crystal structure of Uronate dehydrogenase from Agrobacterium tumefaciens complexed with NADH and productCrystal structure of Uronate dehydrogenase from Agrobacterium tumefaciens complexed with NADH and product

Structural highlights

3rfv is a 3 chain structure with sequence from Agrobacterium fabrum str. C58. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.1Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

URODH_AGRFC Catalyzes the oxidation of D-galacturonate and D-glucuronate to galactarate and D-glucarate, respectively. In fact, in water solution the substrate D-galacturonate is predominantly in pyranosic form whose beta anomer is converted by the enzyme to D-galactaro-1,5-lactone; in solution, this reaction product rearranges to the more stable D-galactaro-1,4-lactone. Makes part of the oxidative degradation pathway of D-galacturonate, which allows A.tumefaciens to utilize D-galacturonate as a sole carbon source. Cannot use NADP(+) instead of NAD(+) as cosubstrate. Is not active on D-galactose, D-glucose, D-galactonate and D-gluconate.^[1] ^[2] ^[3]

References

↑ Yoon SH, Moon TS, Iranpour P, Lanza AM, Prather KJ. Cloning and characterization of uronate dehydrogenases from two pseudomonads and Agrobacterium tumefaciens strain C58. J Bacteriol. 2009 Mar;191(5):1565-73. PMID:19060141 doi:10.1128/JB.00586-08
↑ Boer H, Maaheimo H, Koivula A, Penttilä M, Richard P. Identification in Agrobacterium tumefaciens of the D-galacturonic acid dehydrogenase gene. Appl Microbiol Biotechnol. 2010 Apr;86(3):901-9. PMID:19921179 doi:10.1007/s00253-009-2333-9
↑ Parkkinen T, Boer H, Janis J, Andberg M, Penttila M, Koivula A, Rouvinen J. Crystal structure of uronate dehydrogenase from Agrobacterium tumefaciens. J Biol Chem. 2011 Jun 15. PMID:21676870 doi:10.1074/jbc.M111.254854

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OCA

[1] Yoon SH, Moon TS, Iranpour P, Lanza AM, Prather KJ. Cloning and characterization of uronate dehydrogenases from two pseudomonads and Agrobacterium tumefaciens strain C58. J Bacteriol. 2009 Mar;191(5):1565-73. PMID:19060141 doi:10.1128/JB.00586-08

[2] Boer H, Maaheimo H, Koivula A, Penttilä M, Richard P. Identification in Agrobacterium tumefaciens of the D-galacturonic acid dehydrogenase gene. Appl Microbiol Biotechnol. 2010 Apr;86(3):901-9. PMID:19921179 doi:10.1007/s00253-009-2333-9

[3] Parkkinen T, Boer H, Janis J, Andberg M, Penttila M, Koivula A, Rouvinen J. Crystal structure of uronate dehydrogenase from Agrobacterium tumefaciens. J Biol Chem. 2011 Jun 15. PMID:21676870 doi:10.1074/jbc.M111.254854

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