3r1x
Crystal structure of 2-oxo-3-deoxygalactonate kinase from Klebsiella pneumoniaeCrystal structure of 2-oxo-3-deoxygalactonate kinase from Klebsiella pneumoniae
Structural highlights
Publication Abstract from PubMedIn most organisms, efficient D-galactose utilization requires the highly conserved Leloir pathway that converts D-galactose to D-glucose 1-phosphate. However, in some bacterial and fungal species alternative routes of D-galactose assimilation have been identified. In the so-called De Ley-Doudoroff pathway, D-galactose is metabolized into pyruvate and D-glyceraldehyde 3-phosphate in five consecutive reactions carried out by specific enzymes. The penultimate step in this pathway involves the phosphorylation of 2-oxo-3-deoxygalactonate to 2-oxo-3-deoxygalactonate 6-phosphate catalyzed by 2-oxo-3-deoxygalactonate kinase, with ATP serving as a phosphoryl-group donor. Here, a crystal structure of 2-oxo-3-deoxygalactonate kinase from Klebsiella pneumoniae determined at 2.1 A resolution is reported, the first structure of an enzyme from the De Ley-Doudoroff pathway. Structural comparison indicates that the enzyme belongs to the ASKHA (acetate and sugar kinases/hsc70/actin) family of phosphotransferases. The protein is composed of two alpha/beta domains, each of which contains a core common to all family members. Additional elements introduced between conserved structural motifs define the unique features of 2-oxo-3-deoxygalactonate kinase and possibly determine the biological function of the protein. Structure of 2-oxo-3-deoxygalactonate kinase from Klebsiella pneumoniae.,Michalska K, Cuff ME, Tesar C, Feldmann B, Joachimiak A Acta Crystallogr D Biol Crystallogr. 2011 Aug;67(Pt 8):678-89. Epub 2011, Jul 12. PMID:21795809[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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