3prx

Structure of Complement C5 in Complex with CVF and SSL7Structure of Complement C5 in Complex with CVF and SSL7

Structural highlights 3prx is a 6 chain structure with sequence from Homo sapiens, Naja kaouthia and Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 4.3Å Ligands: Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function D3JIB2_STAAU Publication Abstract from PubMed Complement acts as a danger-sensing system in the innate immune system, and its activation initiates a strong inflammatory response and cleavage of the proteins C3 and C5 by proteolytic enzymes, the convertases. These contain a non-catalytic substrate contacting subunit (C3b or C4b) in complex with a protease subunit (Bb or C2a). We determined the crystal structures of the C3b homologue cobra venom factor (CVF) in complex with C5, and in complex with C5 and the inhibitor SSL7 at 4.3 A resolution. The structures reveal a parallel two-point attachment between C5 and CVF, where the presence of SSL7 only slightly affects the C5-CVF interface, explaining the IgA dependence for SSL7-mediated inhibition of C5 cleavage. CVF functions as a relatively rigid binding scaffold inducing a conformational change in C5, which positions its cleavage site in proximity to the serine protease Bb. A general model for substrate recognition by the convertases is presented based on the C5-CVF and C3b-Bb-SCIN structures. Prior knowledge concerning interactions between the endogenous convertases and their substrates is rationalized by this model. Substrate recognition by complement convertases revealed in the C5-cobra venom factor complex.,Laursen NS, Andersen KR, Braren I, Spillner E, Sottrup-Jensen L, Andersen GR EMBO J. 2011 Jan 7. PMID:21217642 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Cobra venom factor Complement C5 3D structures

spinqualitylabelsall models 3prx, resolution 4.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image