3n0g

Crystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens) in complex with three Mg2+ ions and dimethylallyl-S-thiolodiphosphateCrystal Structure of Isoprene Synthase from Grey Poplar Leaves (Populus x canescens) in complex with three Mg2+ ions and dimethylallyl-S-thiolodiphosphate

Structural highlights

3n0g is a 2 chain structure with sequence from Populus tremula x Populus alba. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.8Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ISPS_POPCN Lyase that catalyzes the formation of isoprene from dimethylallyl diphosphate via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The X-ray crystal structure of recombinant PcISPS (isoprene synthase from gray poplar hybrid Populusxcanescens) has been determined at 2.7 A resolution, and the structure of its complex with three Mg(2+) and the unreactive substrate analogue dimethylallyl-S-thiolodiphosphate has been determined at 2.8 A resolution. Analysis of these structures suggests that the generation of isoprene from substrate dimethylallyl diphosphate occurs via a syn-periplanar elimination mechanism in which the diphosphate-leaving group serves as a general base. This chemical mechanism is responsible for the annual atmospheric emission of 100 Tg of isoprene by terrestrial plant life. Importantly, the PcISPS structure promises to guide future protein engineering studies, potentially leading to hydrocarbon fuels and products that do not rely on traditional petrochemical sources.

Structure of Isoprene Synthase Illuminates the Chemical Mechanism of Teragram Atmospheric Carbon Emission.,Koksal M, Zimmer I, Schnitzler JP, Christianson DW J Mol Biol. 2010 Jul 17. PMID:20624401^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Miller B, Oschinski C, Zimmer W. First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli. Planta. 2001 Jul;213(3):483-7. PMID:11506373 doi:10.1007/s004250100557
↑ Koksal M, Zimmer I, Schnitzler JP, Christianson DW. Structure of Isoprene Synthase Illuminates the Chemical Mechanism of Teragram Atmospheric Carbon Emission. J Mol Biol. 2010 Jul 17. PMID:20624401 doi:10.1016/j.jmb.2010.07.009
↑ Koksal M, Zimmer I, Schnitzler JP, Christianson DW. Structure of Isoprene Synthase Illuminates the Chemical Mechanism of Teragram Atmospheric Carbon Emission. J Mol Biol. 2010 Jul 17. PMID:20624401 doi:10.1016/j.jmb.2010.07.009

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OCA

[1] Miller B, Oschinski C, Zimmer W. First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli. Planta. 2001 Jul;213(3):483-7. PMID:11506373 doi:10.1007/s004250100557

[2] Koksal M, Zimmer I, Schnitzler JP, Christianson DW. Structure of Isoprene Synthase Illuminates the Chemical Mechanism of Teragram Atmospheric Carbon Emission. J Mol Biol. 2010 Jul 17. PMID:20624401 doi:10.1016/j.jmb.2010.07.009

[3] Koksal M, Zimmer I, Schnitzler JP, Christianson DW. Structure of Isoprene Synthase Illuminates the Chemical Mechanism of Teragram Atmospheric Carbon Emission. J Mol Biol. 2010 Jul 17. PMID:20624401 doi:10.1016/j.jmb.2010.07.009

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