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Structure of the Urease Accessory Protein UreF from Helicobacter pyloriStructure of the Urease Accessory Protein UreF from Helicobacter pylori
Structural highlights
FunctionUREF_HELPY Required for maturation of urease via the functional incorporation of the urease nickel metallocenter (By similarity).[HAMAP-Rule:MF_01385] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedUrease plays a central role in the pathogenesis of Helicobacter pylori in humans. Maturation of this nickel metalloenzyme in bacteria requires the participation of the accessory proteins UreD (termed UreH in H. pylori), UreF, and UreG, which form sequential complexes with the urease apoprotein as well as UreE, a metallochaperone. Here, we describe the crystal structure of C-terminal truncated UreF from H. pylori (residues 1-233), the first UreF structure to be determined, at 1.55 A resolution using SAD methods. UreF forms a dimer in vitro and adopts an all-helical fold congruent with secondary structure prediction. On the basis of evolutionary conservation analysis, the structure reveals a probable binding surface for interaction with other urease components as well as key conserved residues of potential functional relevance. Proteins 2010. (c) 2010 Wiley-Liss, Inc. Crystal structure of a truncated urease accessory protein UreF from Helicobacter pylori.,Lam R, Romanov V, Johns K, Battaile KP, Wu-Brown J, Guthrie JL, Hausinger RP, Pai EF, Chirgadze NY Proteins. 2010 Jun 14. PMID:20635345[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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