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Crystal Structure of Parasite Sarcocystis muris Microneme Protein SML- 2 in complex with 1-Thio-beta-D-Galactose (SPACEGROUP C2221)Crystal Structure of Parasite Sarcocystis muris Microneme Protein SML- 2 in complex with 1-Thio-beta-D-Galactose (SPACEGROUP C2221)
Structural highlights
FunctionMIA2_SARMU Galactose-binding lectin. Plays a role in adhesion to the host cell. Has a potential role in invasion of host cells.[1] [2] Publication Abstract from PubMedThe microneme protein SML-2 is a member of a small family of galactose-specific lectins that play a role during host-cell invasion by the apicomplexan parasite Sarcocystis muris. The structures of apo SML-2 and the 1-thio-beta-D-galactose-SML-2 complex were determined at 1.95 and 2.1 A resolution, respectively, by sulfur-SAD phasing. Highly elongated dimers are formed by PAN-domain tandems in the protomer, bearing the galactose-binding cavities at the distal apple-like domains. The detailed structure of the binding site in SML-2 explains the high specificity of galactose-endgroup binding and the broader specificity of the related Toxoplasma gondii protein TgMIC4 towards galactose and glucose. A large buried surface of highly hydrophobic character and 24 intersubunit hydrogen bonds stabilize the dimers and half of the 12 disulfides per dimer are shielded from the solvent by the polypeptide chain, thereby enhancing the resistance of the parasite protein towards unfolding and proteolysis that allows it to survive within the intestinal tracts of the intermediate and final hosts. PAN-modular structure of microneme protein SML-2 from the parasite Sarcocystis muris at 1.95 A resolution and its complex with 1-thio-beta-D-galactose.,Muller JJ, Weiss MS, Heinemann U Acta Crystallogr D Biol Crystallogr. 2011 Nov;67(Pt 11):936-44. Epub 2011, Oct 19. PMID:22101820[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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