Proteopedia

2vkn

YEAST SHO1 SH3 DOMAIN COMPLEXED WITH A PEPTIDE FROM PBS2YEAST SHO1 SH3 DOMAIN COMPLEXED WITH A PEPTIDE FROM PBS2

Structural highlights

2vkn is a 2 chain structure with sequence from Saccharomyces cerevisiae. This structure supersedes the now removed PDB entry 2qk6. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.05Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SHO1_YEAST Plasma membrane osmosensor that activates the high osmolarity glycerol (HOG) MAPK signaling pathway in response to high osmolarity. Detects changes in external osmolarity and activates PBS2 through the stimulation of STE11 and targets PBS2 to the plasma membrane. PBS2 activation leads to changes in glycerol production that helps to balance the intracellular and external osmotic pressures. Activates also HOG1 in response to heat stress and mediates resistance to oxidative stress. Involved in the regulation of the mating pathway. May be a receptor that feeds into the pseudohyphal growth pathway.[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20]

Evolutionary Conservation

 

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Garcia-Rodriguez LJ, Duran A, Roncero C. Calcofluor antifungal action depends on chitin and a functional high-osmolarity glycerol response (HOG) pathway: evidence for a physiological role of the Saccharomyces cerevisiae HOG pathway under noninducing conditions. J Bacteriol. 2000 May;182(9):2428-37. PMID:10762242
  2. ↑ Van Wuytswinkel O, Reiser V, Siderius M, Kelders MC, Ammerer G, Ruis H, Mager WH. Response of Saccharomyces cerevisiae to severe osmotic stress: evidence for a novel activation mechanism of the HOG MAP kinase pathway. Mol Microbiol. 2000 Jul;37(2):382-97. PMID:10931333
  3. ↑ Raitt DC, Posas F, Saito H. Yeast Cdc42 GTPase and Ste20 PAK-like kinase regulate Sho1-dependent activation of the Hog1 MAPK pathway. EMBO J. 2000 Sep 1;19(17):4623-31. PMID:10970855 doi:http://dx.doi.org/10.1093/emboj/19.17.4623
  4. ↑ Reiser V, Salah SM, Ammerer G. Polarized localization of yeast Pbs2 depends on osmostress, the membrane protein Sho1 and Cdc42. Nat Cell Biol. 2000 Sep;2(9):620-7. PMID:10980703 doi:http://dx.doi.org/10.1038/35023568
  5. ↑ Singh KK. The Saccharomyces cerevisiae Sln1p-Ssk1p two-component system mediates response to oxidative stress and in an oxidant-specific fashion. Free Radic Biol Med. 2000 Nov 15;29(10):1043-50. PMID:11084293
  6. ↑ Toh-e A, Oguchi T. Defects in glycosylphosphatidylinositol (GPI) anchor synthesis activate Hog1 kinase and confer copper-resistance in Saccharomyces cerevisisae. Genes Genet Syst. 2001 Dec;76(6):393-410. PMID:11922108
  7. ↑ Winkler A, Arkind C, Mattison CP, Burkholder A, Knoche K, Ota I. Heat stress activates the yeast high-osmolarity glycerol mitogen-activated protein kinase pathway, and protein tyrosine phosphatases are essential under heat stress. Eukaryot Cell. 2002 Apr;1(2):163-73. PMID:12455951
  8. ↑ Park SH, Zarrinpar A, Lim WA. Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms. Science. 2003 Feb 14;299(5609):1061-4. Epub 2003 Jan 2. PMID:12511654 doi:http://dx.doi.org/10.1126/science.1076979
  9. ↑ O'Rourke SM, Herskowitz I. Unique and redundant roles for HOG MAPK pathway components as revealed by whole-genome expression analysis. Mol Biol Cell. 2004 Feb;15(2):532-42. Epub 2003 Oct 31. PMID:14595107 doi:http://dx.doi.org/10.1091/mbc.E03-07-0521
  10. ↑ Nelson B, Parsons AB, Evangelista M, Schaefer K, Kennedy K, Ritchie S, Petryshen TL, Boone C. Fus1p interacts with components of the Hog1p mitogen-activated protein kinase and Cdc42p morphogenesis signaling pathways to control cell fusion during yeast mating. Genetics. 2004 Jan;166(1):67-77. PMID:15020407
  11. ↑ Marles JA, Dahesh S, Haynes J, Andrews BJ, Davidson AR. Protein-protein interaction affinity plays a crucial role in controlling the Sho1p-mediated signal transduction pathway in yeast. Mol Cell. 2004 Jun 18;14(6):813-23. PMID:15200958 doi:http://dx.doi.org/10.1016/j.molcel.2004.05.024
  12. ↑ Zarrinpar A, Bhattacharyya RP, Nittler MP, Lim WA. Sho1 and Pbs2 act as coscaffolds linking components in the yeast high osmolarity MAP kinase pathway. Mol Cell. 2004 Jun 18;14(6):825-32. PMID:15200959 doi:http://dx.doi.org/10.1016/j.molcel.2004.06.011
  13. ↑ Cullen PJ, Sabbagh W Jr, Graham E, Irick MM, van Olden EK, Neal C, Delrow J, Bardwell L, Sprague GF Jr. A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast. Genes Dev. 2004 Jul 15;18(14):1695-708. PMID:15256499 doi:http://dx.doi.org/10.1101/gad.1178604
  14. ↑ Tatebayashi K, Yamamoto K, Tanaka K, Tomida T, Maruoka T, Kasukawa E, Saito H. Adaptor functions of Cdc42, Ste50, and Sho1 in the yeast osmoregulatory HOG MAPK pathway. EMBO J. 2006 Jul 12;25(13):3033-44. Epub 2006 Jun 15. PMID:16778768 doi:http://dx.doi.org/10.1038/sj.emboj.7601192
  15. ↑ Hersen P, McClean MN, Mahadevan L, Ramanathan S. Signal processing by the HOG MAP kinase pathway. Proc Natl Acad Sci U S A. 2008 May 20;105(20):7165-70. doi:, 10.1073/pnas.0710770105. Epub 2008 May 14. PMID:18480263 doi:http://dx.doi.org/10.1073/pnas.0710770105
  16. ↑ Macia J, Regot S, Peeters T, Conde N, Sole R, Posas F. Dynamic signaling in the Hog1 MAPK pathway relies on high basal signal transduction. Sci Signal. 2009 Mar 24;2(63):ra13. doi: 10.1126/scisignal.2000056. PMID:19318625 doi:http://dx.doi.org/10.1126/scisignal.2000056
  17. ↑ Pitoniak A, Birkaya B, Dionne HM, Vadaie N, Cullen PJ. The signaling mucins Msb2 and Hkr1 differentially regulate the filamentation mitogen-activated protein kinase pathway and contribute to a multimodal response. Mol Biol Cell. 2009 Jul;20(13):3101-14. Epub 2009 May 13. PMID:19439450 doi:http://dx.doi.org/E08-07-0760
  18. ↑ Maeda T, Takekawa M, Saito H. Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor. Science. 1995 Jul 28;269(5223):554-8. PMID:7624781
  19. ↑ Posas F, Saito H. Osmotic activation of the HOG MAPK pathway via Ste11p MAPKKK: scaffold role of Pbs2p MAPKK. Science. 1997 Jun 13;276(5319):1702-5. PMID:9180081
  20. ↑ O'Rourke SM, Herskowitz I. The Hog1 MAPK prevents cross talk between the HOG and pheromone response MAPK pathways in Saccharomyces cerevisiae. Genes Dev. 1998 Sep 15;12(18):2874-86. PMID:9744864

2vkn, resolution 2.05Å

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