2q3h

The crystal structure of RhouA in the GDP-bound state.The crystal structure of RhouA in the GDP-bound state.

Structural highlights

2q3h is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.73Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RHOU_HUMAN Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.^[1] ^[2] ^[3] ^[4] ^[5]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Tao W, Pennica D, Xu L, Kalejta RF, Levine AJ. Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1. Genes Dev. 2001 Jul 15;15(14):1796-807. PMID:11459829 doi:10.1101/gad.894301
↑ Shutes A, Berzat AC, Chenette EJ, Cox AD, Der CJ. Biochemical analyses of the Wrch atypical Rho family GTPases. Methods Enzymol. 2006;406:11-26. PMID:16472646 doi:10.1016/S0076-6879(06)06002-2
↑ Ory S, Brazier H, Blangy A. Identification of a bipartite focal adhesion localization signal in RhoU/Wrch-1, a Rho family GTPase that regulates cell adhesion and migration. Biol Cell. 2007 Dec;99(12):701-16. PMID:17620058 doi:10.1042/BC20070058
↑ Ruusala A, Aspenstrom P. The atypical Rho GTPase Wrch1 collaborates with the nonreceptor tyrosine kinases Pyk2 and Src in regulating cytoskeletal dynamics. Mol Cell Biol. 2008 Mar;28(5):1802-14. Epub 2007 Dec 17. PMID:18086875 doi:10.1128/MCB.00201-07
↑ Bai SW, Herrera-Abreu MT, Rohn JL, Racine V, Tajadura V, Suryavanshi N, Bechtel S, Wiemann S, Baum B, Ridley AJ. Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. BMC Biol. 2011 Aug 11;9:54. doi: 10.1186/1741-7007-9-54. PMID:21834987 doi:10.1186/1741-7007-9-54

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OCA

[1] Tao W, Pennica D, Xu L, Kalejta RF, Levine AJ. Wrch-1, a novel member of the Rho gene family that is regulated by Wnt-1. Genes Dev. 2001 Jul 15;15(14):1796-807. PMID:11459829 doi:10.1101/gad.894301

[2] Shutes A, Berzat AC, Chenette EJ, Cox AD, Der CJ. Biochemical analyses of the Wrch atypical Rho family GTPases. Methods Enzymol. 2006;406:11-26. PMID:16472646 doi:10.1016/S0076-6879(06)06002-2

[3] Ory S, Brazier H, Blangy A. Identification of a bipartite focal adhesion localization signal in RhoU/Wrch-1, a Rho family GTPase that regulates cell adhesion and migration. Biol Cell. 2007 Dec;99(12):701-16. PMID:17620058 doi:10.1042/BC20070058

[4] Ruusala A, Aspenstrom P. The atypical Rho GTPase Wrch1 collaborates with the nonreceptor tyrosine kinases Pyk2 and Src in regulating cytoskeletal dynamics. Mol Cell Biol. 2008 Mar;28(5):1802-14. Epub 2007 Dec 17. PMID:18086875 doi:10.1128/MCB.00201-07

[5] Bai SW, Herrera-Abreu MT, Rohn JL, Racine V, Tajadura V, Suryavanshi N, Bechtel S, Wiemann S, Baum B, Ridley AJ. Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. BMC Biol. 2011 Aug 11;9:54. doi: 10.1186/1741-7007-9-54. PMID:21834987 doi:10.1186/1741-7007-9-54

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