2lpw

human CEB25 minisatellite G-quadruplexhuman CEB25 minisatellite G-quadruplex

Structural highlights 2lpw is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed CEB25 is a human minisatellite locus, composed of slightly polymorphic 52-nucleotide (nt) tandem repeats. Genetically, most if not all individuals of the human population are heterozygous, carrying alleles ranging from 0.5 to 20 kb, maintained by mendelian inheritance but also subject to germline instability. To provide insights on the biological role of CEB25, we interrogated its structural features. We report the NMR structure of the G-quadruplex formed by the conserved 26-nt G-rich fragment of the CEB25 motif. In K(+) solution, this sequence forms a propeller-type parallel-stranded G-quadruplex involving a 9-nt central double-chain-reversal loop. This long loop is anchored to the 5'-end of the sequence by an A.T Watson-Crick base pair and a potential G.A noncanonical base pair. These base pairs contribute to the stability of the overall G-quadruplexstructure, as measured by an increase of about 17 kcal/mol in enthalpy or 6 degrees C in melting temperature. Further, we demonstrate that such a monomorphic structure is formed within longer sequence contexts folding into a pearl-necklace structure. Formation of Pearl-Necklace Monomorphic G-Quadruplexes in the Human CEB25 Minisatellite.,Amrane S, Adrian M, Heddi B, Serero A, Nicolas A, Mergny JL, Phan AT J Am Chem Soc. 2012 Apr 4;134(13):5807-16. Epub 2012 Mar 26. PMID:22376028 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 2lpw Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image