2ktc

Solution Structure of a Novel hKv1.1 inhibiting scorpion toxin from Mesibuthus tamulusSolution Structure of a Novel hKv1.1 inhibiting scorpion toxin from Mesibuthus tamulus

Structural highlights 2ktc is a 1 chain structure with sequence from Mesobuthus tamulus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR, 20 models Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function KAX94_HOTTA Blocker of human voltage-gated potassium channel Kv1.1/KCNA1 (PubMed:12650917). Publication Abstract from PubMed The three dimensional structure of a 32 residue three disulfide scorpion toxin, BTK-2, from the Indian red scorpion Mesobuthus tamulus has been determined using isotope edited solution NMR methods. Samples for structural and electrophysiological studies were prepared using recombinant DNA methods. Electrophysiological studies show that the peptide is active against hK(v)1.1 channels. The structure of BTK-2 was determined using 373 distance restraints from NOE data, 66 dihedral angle restraints from NOE, chemical shift and scalar coupling data, 6 constraints based on disulfide linkages and 8 constraints based on hydrogen bonds. The root mean square deviation (r.m.s.d) about the averaged co-ordinates of the backbone (N, C(alpha), C') and all heavy atoms are 0.81 +/- 0.23A and 1.51 +/- 0.29A respectively. The backbone dihedral angles (varphi and psi) for all residues occupy the favorable and allowed regions of the Ramachandran map. The three dimensional structure of BTK-2 is composed of three well defined secondary structural regions that constitute the alpha-beta-beta structural motif. Comparisons between the structure of BTK-2 and other closely related scorpion toxins pointed towards distinct differences in surface properties that provide insights into the structure-function relationships among this important class of voltage-gated potassium channel inhibiting peptides. Solution structure of BTK-2, a novel hK(v)1.1 inhibiting scorpion toxin, from the eastern Indian scorpion Mesobuthus tamulus.,Kumar GS, Upadhyay S, Mathew MK, Sarma SP Biochim Biophys Acta. 2011 Apr;1814(4):459-69. doi: 10.1016/j.bbapap.2011.01.006., Epub 2011 Jan 21. PMID:21256986 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Potassium channel toxin 3D structures

spinqualitylabelsall models 2ktc Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image