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The Solution Structure of gpV, the Major Tail Protein from Bacteriophage LambdaThe Solution Structure of gpV, the Major Tail Protein from Bacteriophage Lambda
Structural highlights
FunctionTUBE_LAMBD Forms the phage's tail tube composed of 32 hexameric disks. When it encounters the appropriate initiation complex gpM and gpL, it assembles in hexameric rings that stack on top of each others. Multimerization ceases when the correct tail length is achieved through a mechanism dependent on tail terminator protein.[1] [2] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedMost bacteriophages possess long tails, which serve as the conduit for genome delivery. We report the solution structure of the N-terminal domain of gpV, the protein comprising the major portion of the noncontractile phage lambda tail tube. This structure is very similar to a previously solved tail tube protein from a contractile-tailed phage, providing the first direct evidence of an evolutionary connection between these 2 distinct types of phage tails. A remarkable structural similarity is also seen to Hcp1, a component of the bacterial type VI secretion system. The hexameric structure of Hcp1 and its ability to form long tubes are strikingly reminiscent of gpV when it is polymerized into a tail tube. These data coupled with other similarities between phage and type VI secretion proteins support an evolutionary relationship between these systems. Using Hcp1 as a model, we propose a polymerization mechanism for gpV involving several disorder-to-order transitions. The phage lambda major tail protein structure reveals a common evolution for long-tailed phages and the type VI bacterial secretion system.,Pell LG, Kanelis V, Donaldson LW, Howell PL, Davidson AR Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4160-5. Epub 2009 Feb 27. PMID:19251647[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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