1cld

DNA-binding proteinDNA-binding protein

Structural highlights 1cld is a 1 chain structure with sequence from Kluyveromyces lactis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Ligands: Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function LAC9_KLULA Positive regulatory protein, that controls induction of the lactose-galactose regulation of Kluyveromyces lactis. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding. Solution structure of the Kluyveromyces lactis LAC9 Cd2 Cys6 DNA-binding domain.,Gardner KH, Anderson SF, Coleman JE Nat Struct Biol. 1995 Oct;2(10):898-905. PMID:7552715 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also CD2

spinqualitylabelsall models 1cld Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image