1c1k

BACTERIOPHAGE T4 GENE 59 HELICASE ASSEMBLY PROTEINBACTERIOPHAGE T4 GENE 59 HELICASE ASSEMBLY PROTEIN

Structural highlights

1c1k is a 1 chain structure with sequence from Escherichia virus T4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.45Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HLOAD_BPT4 DNA helicase loader protein that participates in viral DNA replication, recombination, and repair (PubMed:10871615). At the fork, required for loading of the replicative helicase onto DNA protected by the ssDNA-binding protein (PubMed:11459967, PubMed:7806533, PubMed:10871615, PubMed:22427673). Coordinates simultaneous synthesis of leading- and lagging-strands (PubMed:11459969).[HAMAP-Rule:MF_04156]^[1] ^[2] ^[3] ^[4] ^[5]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Jones CE, Mueser TC, Nossal NG. Interaction of the bacteriophage T4 gene 59 helicase loading protein and gene 41 helicase with each other and with fork, flap, and cruciform DNA. J Biol Chem. 2000 Sep 1;275(35):27145-54. PMID:10871615 doi:10.1074/jbc.M003808200
↑ Bleuit JS, Xu H, Ma Y, Wang T, Liu J, Morrical SW. Mediator proteins orchestrate enzyme-ssDNA assembly during T4 recombination-dependent DNA replication and repair. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8298-305. PMID:11459967 doi:10.1073/pnas.131007498
↑ Dolezal D, Jones CE, Lai X, Brister JR, Mueser TC, Nossal NG, Hinton DM. Mutational analysis of the T4 gp59 helicase loader reveals its sites for interaction with helicase, single-stranded binding protein, and DNA. J Biol Chem. 2012 May 25;287(22):18596-607. PMID:22427673 doi:10.1074/jbc.M111.332080
↑ Barry J, Alberts B. Purification and characterization of bacteriophage T4 gene 59 protein. A DNA helicase assembly protein involved in DNA replication. J Biol Chem. 1994 Dec 30;269(52):33049-62 PMID:7806533
↑ Jones CE, Mueser TC, Dudas KC, Kreuzer KN, Nossal NG. Bacteriophage T4 gene 41 helicase and gene 59 helicase-loading protein: a versatile couple with roles in replication and recombination. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8312-8. PMID:11459969 doi:10.1073/pnas.121009398

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OCA

[1] Jones CE, Mueser TC, Nossal NG. Interaction of the bacteriophage T4 gene 59 helicase loading protein and gene 41 helicase with each other and with fork, flap, and cruciform DNA. J Biol Chem. 2000 Sep 1;275(35):27145-54. PMID:10871615 doi:10.1074/jbc.M003808200

[2] Bleuit JS, Xu H, Ma Y, Wang T, Liu J, Morrical SW. Mediator proteins orchestrate enzyme-ssDNA assembly during T4 recombination-dependent DNA replication and repair. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8298-305. PMID:11459967 doi:10.1073/pnas.131007498

[3] Dolezal D, Jones CE, Lai X, Brister JR, Mueser TC, Nossal NG, Hinton DM. Mutational analysis of the T4 gp59 helicase loader reveals its sites for interaction with helicase, single-stranded binding protein, and DNA. J Biol Chem. 2012 May 25;287(22):18596-607. PMID:22427673 doi:10.1074/jbc.M111.332080

[4] Barry J, Alberts B. Purification and characterization of bacteriophage T4 gene 59 protein. A DNA helicase assembly protein involved in DNA replication. J Biol Chem. 1994 Dec 30;269(52):33049-62 PMID:7806533

[5] Jones CE, Mueser TC, Dudas KC, Kreuzer KN, Nossal NG. Bacteriophage T4 gene 41 helicase and gene 59 helicase-loading protein: a versatile couple with roles in replication and recombination. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8312-8. PMID:11459969 doi:10.1073/pnas.121009398

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